Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC...

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Autores principales: Tang, Ngang Heok, Okada, Naoyuki, Fong, Chii Shyang, Arai, Kunio, Sato, Masamitsu, Toda, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Ltd 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158419/
https://www.ncbi.nlm.nih.gov/pubmed/24937146
http://dx.doi.org/10.1016/j.febslet.2014.06.027
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author Tang, Ngang Heok
Okada, Naoyuki
Fong, Chii Shyang
Arai, Kunio
Sato, Masamitsu
Toda, Takashi
author_facet Tang, Ngang Heok
Okada, Naoyuki
Fong, Chii Shyang
Arai, Kunio
Sato, Masamitsu
Toda, Takashi
author_sort Tang, Ngang Heok
collection PubMed
description The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.
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spelling pubmed-41584192014-09-10 Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast Tang, Ngang Heok Okada, Naoyuki Fong, Chii Shyang Arai, Kunio Sato, Masamitsu Toda, Takashi FEBS Lett Article The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform. John Wiley & Sons Ltd 2014-08-25 /pmc/articles/PMC4158419/ /pubmed/24937146 http://dx.doi.org/10.1016/j.febslet.2014.06.027 Text en Crown Copyright © 2014 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Tang, Ngang Heok
Okada, Naoyuki
Fong, Chii Shyang
Arai, Kunio
Sato, Masamitsu
Toda, Takashi
Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
title Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
title_full Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
title_fullStr Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
title_full_unstemmed Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
title_short Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
title_sort targeting alp7/tacc to the spindle pole body is essential for mitotic spindle assembly in fission yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158419/
https://www.ncbi.nlm.nih.gov/pubmed/24937146
http://dx.doi.org/10.1016/j.febslet.2014.06.027
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