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Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells
Interleukin-8 (IL-8) plays key roles in both chronic inflammatory diseases and tumor modulation. We previously observed that IL-8 secretion and function can be modulated by nucleotide (P2) receptors. Here we investigated whether IL-8 release by intestinal epithelial HT-29 cells, a cancer cell line,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158428/ https://www.ncbi.nlm.nih.gov/pubmed/25242873 http://dx.doi.org/10.1155/2014/879895 |
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author | Bahrami, Fariborz Kukulski, Filip Lecka, Joanna Tremblay, Alain Pelletier, Julie Rockenbach, Liliana Sévigny, Jean |
author_facet | Bahrami, Fariborz Kukulski, Filip Lecka, Joanna Tremblay, Alain Pelletier, Julie Rockenbach, Liliana Sévigny, Jean |
author_sort | Bahrami, Fariborz |
collection | PubMed |
description | Interleukin-8 (IL-8) plays key roles in both chronic inflammatory diseases and tumor modulation. We previously observed that IL-8 secretion and function can be modulated by nucleotide (P2) receptors. Here we investigated whether IL-8 release by intestinal epithelial HT-29 cells, a cancer cell line, is modulated by extracellular nucleotide metabolism. We first identified that HT-29 cells regulated adenosine and adenine nucleotide concentration at their surface by the expression of the ectoenzymes NTPDase2, ecto-5′-nucleotidase, and adenylate kinase. The expression of the ectoenzymes was evaluated by RT-PCR, qPCR, and immunoblotting, and their activity was analyzed by RP-HPLC of the products and by detection of P(i) produced from the hydrolysis of ATP, ADP, and AMP. In response to poly (I:C), with or without ATP and/or ADP, HT-29 cells released IL-8 and this secretion was modulated by the presence of NTPDase2 and adenylate kinase. Taken together, these results demonstrate the presence of 3 ectoenzymes at the surface of HT-29 cells that control nucleotide levels and adenosine production (NTPDase2, ecto-5′-nucleotidase and adenylate kinase) and that P2 receptor-mediated signaling controls IL-8 release in HT-29 cells which is modulated by the presence of NTPDase2 and adenylate kinase. |
format | Online Article Text |
id | pubmed-4158428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41584282014-09-21 Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells Bahrami, Fariborz Kukulski, Filip Lecka, Joanna Tremblay, Alain Pelletier, Julie Rockenbach, Liliana Sévigny, Jean Mediators Inflamm Research Article Interleukin-8 (IL-8) plays key roles in both chronic inflammatory diseases and tumor modulation. We previously observed that IL-8 secretion and function can be modulated by nucleotide (P2) receptors. Here we investigated whether IL-8 release by intestinal epithelial HT-29 cells, a cancer cell line, is modulated by extracellular nucleotide metabolism. We first identified that HT-29 cells regulated adenosine and adenine nucleotide concentration at their surface by the expression of the ectoenzymes NTPDase2, ecto-5′-nucleotidase, and adenylate kinase. The expression of the ectoenzymes was evaluated by RT-PCR, qPCR, and immunoblotting, and their activity was analyzed by RP-HPLC of the products and by detection of P(i) produced from the hydrolysis of ATP, ADP, and AMP. In response to poly (I:C), with or without ATP and/or ADP, HT-29 cells released IL-8 and this secretion was modulated by the presence of NTPDase2 and adenylate kinase. Taken together, these results demonstrate the presence of 3 ectoenzymes at the surface of HT-29 cells that control nucleotide levels and adenosine production (NTPDase2, ecto-5′-nucleotidase and adenylate kinase) and that P2 receptor-mediated signaling controls IL-8 release in HT-29 cells which is modulated by the presence of NTPDase2 and adenylate kinase. Hindawi Publishing Corporation 2014 2014-07-23 /pmc/articles/PMC4158428/ /pubmed/25242873 http://dx.doi.org/10.1155/2014/879895 Text en Copyright © 2014 Fariborz Bahrami et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bahrami, Fariborz Kukulski, Filip Lecka, Joanna Tremblay, Alain Pelletier, Julie Rockenbach, Liliana Sévigny, Jean Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells |
title | Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells |
title_full | Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells |
title_fullStr | Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells |
title_full_unstemmed | Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells |
title_short | Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells |
title_sort | purine-metabolizing ectoenzymes control il-8 production in human colon ht-29 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158428/ https://www.ncbi.nlm.nih.gov/pubmed/25242873 http://dx.doi.org/10.1155/2014/879895 |
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