Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway

Keratinocyte migration is an early event in the wound healing process. Although we previously found that CD9 downregulation is required for the keratinocyte migration during wound repair, the mechanism of how CD9 expression is regulated remains unclear. Here, we observed the effect of hypoxia (2% O(...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Xupin, Guo, Xiaowei, Xu, Xue, Teng, Miao, Huang, Chong, Zhang, Dongxia, Zhang, Qiong, Zhang, Jiaping, Huang, Yuesheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158574/
https://www.ncbi.nlm.nih.gov/pubmed/25200404
http://dx.doi.org/10.1038/srep06304
_version_ 1782334080837222400
author Jiang, Xupin
Guo, Xiaowei
Xu, Xue
Teng, Miao
Huang, Chong
Zhang, Dongxia
Zhang, Qiong
Zhang, Jiaping
Huang, Yuesheng
author_facet Jiang, Xupin
Guo, Xiaowei
Xu, Xue
Teng, Miao
Huang, Chong
Zhang, Dongxia
Zhang, Qiong
Zhang, Jiaping
Huang, Yuesheng
author_sort Jiang, Xupin
collection PubMed
description Keratinocyte migration is an early event in the wound healing process. Although we previously found that CD9 downregulation is required for the keratinocyte migration during wound repair, the mechanism of how CD9 expression is regulated remains unclear. Here, we observed the effect of hypoxia (2% O(2)) on CD9 expression and keratinocyte migration. CD9 expression was downregulated and keratinocyte migration was increased under hypoxic conditions. In addition, CD9 overexpression reversed hypoxia-induced cell migration. We also found that hypoxia activated the p38/MAPK pathway. SB203580, a p38/MAPK inhibitor, increased CD9 expression and inhibited keratinocyte migration under hypoxia, while MKK6 (Glu) overexpression decreased CD9 expression and promoted hypoxic keratinocyte migration. Our results demonstrate that hypoxia regulates CD9 expression and CD9-mediated keratinocyte migration via the p38/MAPK pathway.
format Online
Article
Text
id pubmed-4158574
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-41585742014-09-10 Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway Jiang, Xupin Guo, Xiaowei Xu, Xue Teng, Miao Huang, Chong Zhang, Dongxia Zhang, Qiong Zhang, Jiaping Huang, Yuesheng Sci Rep Article Keratinocyte migration is an early event in the wound healing process. Although we previously found that CD9 downregulation is required for the keratinocyte migration during wound repair, the mechanism of how CD9 expression is regulated remains unclear. Here, we observed the effect of hypoxia (2% O(2)) on CD9 expression and keratinocyte migration. CD9 expression was downregulated and keratinocyte migration was increased under hypoxic conditions. In addition, CD9 overexpression reversed hypoxia-induced cell migration. We also found that hypoxia activated the p38/MAPK pathway. SB203580, a p38/MAPK inhibitor, increased CD9 expression and inhibited keratinocyte migration under hypoxia, while MKK6 (Glu) overexpression decreased CD9 expression and promoted hypoxic keratinocyte migration. Our results demonstrate that hypoxia regulates CD9 expression and CD9-mediated keratinocyte migration via the p38/MAPK pathway. Nature Publishing Group 2014-09-09 /pmc/articles/PMC4158574/ /pubmed/25200404 http://dx.doi.org/10.1038/srep06304 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Jiang, Xupin
Guo, Xiaowei
Xu, Xue
Teng, Miao
Huang, Chong
Zhang, Dongxia
Zhang, Qiong
Zhang, Jiaping
Huang, Yuesheng
Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
title Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
title_full Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
title_fullStr Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
title_full_unstemmed Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
title_short Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
title_sort hypoxia regulates cd9-mediated keratinocyte migration via the p38/mapk pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158574/
https://www.ncbi.nlm.nih.gov/pubmed/25200404
http://dx.doi.org/10.1038/srep06304
work_keys_str_mv AT jiangxupin hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT guoxiaowei hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT xuxue hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT tengmiao hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT huangchong hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT zhangdongxia hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT zhangqiong hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT zhangjiaping hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway
AT huangyuesheng hypoxiaregulatescd9mediatedkeratinocytemigrationviathep38mapkpathway

Ejemplares similares