Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()

Polyphenol oxidases are involved in aurone biosynthesis but the gene responsible for 4-deoxyaurone formation in Asteraceae was so far unknown. Three novel full-length cDNA sequences were isolated from Coreopsis grandiflora with sizes of 1.80 kb (cgAUS1) and 1.85 kb (cgAUS2a, 2b), encoding for protei...

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Autores principales: Kaintz, Cornelia, Molitor, Christian, Thill, Jana, Kampatsikas, Ioannis, Michael, Claudia, Halbwirth, Heidi, Rompel, Annette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Ltd 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158910/
https://www.ncbi.nlm.nih.gov/pubmed/25109778
http://dx.doi.org/10.1016/j.febslet.2014.07.034
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author Kaintz, Cornelia
Molitor, Christian
Thill, Jana
Kampatsikas, Ioannis
Michael, Claudia
Halbwirth, Heidi
Rompel, Annette
author_facet Kaintz, Cornelia
Molitor, Christian
Thill, Jana
Kampatsikas, Ioannis
Michael, Claudia
Halbwirth, Heidi
Rompel, Annette
author_sort Kaintz, Cornelia
collection PubMed
description Polyphenol oxidases are involved in aurone biosynthesis but the gene responsible for 4-deoxyaurone formation in Asteraceae was so far unknown. Three novel full-length cDNA sequences were isolated from Coreopsis grandiflora with sizes of 1.80 kb (cgAUS1) and 1.85 kb (cgAUS2a, 2b), encoding for proteins of 68–69 kDa, respectively. cgAUS1 is preferably expressed in young petals indicating a specific role in pigment formation. The 58.9 kDa AUS1 holoproenzyme, was recombinantly expressed in E. coli and purified to homogeneity. The enzyme shows only diphenolase activity, catalyzing the conversion of chalcones to aurones and was characterized by SDS–PAGE and shot-gun type nanoUHPLC–ESI-MS/MS.
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spelling pubmed-41589102014-09-17 Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation() Kaintz, Cornelia Molitor, Christian Thill, Jana Kampatsikas, Ioannis Michael, Claudia Halbwirth, Heidi Rompel, Annette FEBS Lett Article Polyphenol oxidases are involved in aurone biosynthesis but the gene responsible for 4-deoxyaurone formation in Asteraceae was so far unknown. Three novel full-length cDNA sequences were isolated from Coreopsis grandiflora with sizes of 1.80 kb (cgAUS1) and 1.85 kb (cgAUS2a, 2b), encoding for proteins of 68–69 kDa, respectively. cgAUS1 is preferably expressed in young petals indicating a specific role in pigment formation. The 58.9 kDa AUS1 holoproenzyme, was recombinantly expressed in E. coli and purified to homogeneity. The enzyme shows only diphenolase activity, catalyzing the conversion of chalcones to aurones and was characterized by SDS–PAGE and shot-gun type nanoUHPLC–ESI-MS/MS. John Wiley & Sons Ltd 2014-09-17 /pmc/articles/PMC4158910/ /pubmed/25109778 http://dx.doi.org/10.1016/j.febslet.2014.07.034 Text en © 2014 The Authors https://creativecommons.org/licenses/by/3.0/This work is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/) .
spellingShingle Article
Kaintz, Cornelia
Molitor, Christian
Thill, Jana
Kampatsikas, Ioannis
Michael, Claudia
Halbwirth, Heidi
Rompel, Annette
Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()
title Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()
title_full Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()
title_fullStr Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()
title_full_unstemmed Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()
title_short Cloning and functional expression in E. coli of a polyphenol oxidase transcript from Coreopsis grandiflora involved in aurone formation()
title_sort cloning and functional expression in e. coli of a polyphenol oxidase transcript from coreopsis grandiflora involved in aurone formation()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158910/
https://www.ncbi.nlm.nih.gov/pubmed/25109778
http://dx.doi.org/10.1016/j.febslet.2014.07.034
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