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Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis

BACKGROUND: Previous studies of infant fecal samples have failed to clarify the role of gut bacteria in the pathogenesis of NEC. We sought to characterize bacterial communities within intestinal tissue resected from infants with and without NEC. METHODS: 26 intestinal samples were resected from 19 i...

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Autores principales: Brower-Sinning, Rachel, Zhong, Diana, Good, Misty, Firek, Brian, Baker, Robyn, Sodhi, Chhinder P., Hackam, David J., Morowitz, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159227/
https://www.ncbi.nlm.nih.gov/pubmed/25203729
http://dx.doi.org/10.1371/journal.pone.0105046
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author Brower-Sinning, Rachel
Zhong, Diana
Good, Misty
Firek, Brian
Baker, Robyn
Sodhi, Chhinder P.
Hackam, David J.
Morowitz, Michael J.
author_facet Brower-Sinning, Rachel
Zhong, Diana
Good, Misty
Firek, Brian
Baker, Robyn
Sodhi, Chhinder P.
Hackam, David J.
Morowitz, Michael J.
author_sort Brower-Sinning, Rachel
collection PubMed
description BACKGROUND: Previous studies of infant fecal samples have failed to clarify the role of gut bacteria in the pathogenesis of NEC. We sought to characterize bacterial communities within intestinal tissue resected from infants with and without NEC. METHODS: 26 intestinal samples were resected from 19 infants, including 16 NEC samples and 10 non-NEC samples. Bacterial 16S rRNA gene sequences were amplified and sequenced. Analysis allowed for taxonomic identification, and quantitative PCR was used to quantify the bacterial load within samples. RESULTS: NEC samples generally contained an increased total burden of bacteria. NEC and non-NEC sample sets were both marked by high inter-individual variability and an abundance of opportunistic pathogens. There was no statistically significant distinction between the composition of NEC and non-NEC microbial communities. K-means clustering enabled us to identify several stable clusters, including clusters of NEC and midgut volvulus samples enriched with Clostridium and Bacteroides. Another cluster containing both NEC and non-NEC samples was marked by an abundance of Enterobacteriaceae and decreased diversity among NEC samples. CONCLUSIONS: The results indicate that NEC is a disease without a uniform pattern of microbial colonization, but that NEC is associated with an abundance of strict anaerobes and a decrease in community diversity.
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spelling pubmed-41592272014-09-12 Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis Brower-Sinning, Rachel Zhong, Diana Good, Misty Firek, Brian Baker, Robyn Sodhi, Chhinder P. Hackam, David J. Morowitz, Michael J. PLoS One Research Article BACKGROUND: Previous studies of infant fecal samples have failed to clarify the role of gut bacteria in the pathogenesis of NEC. We sought to characterize bacterial communities within intestinal tissue resected from infants with and without NEC. METHODS: 26 intestinal samples were resected from 19 infants, including 16 NEC samples and 10 non-NEC samples. Bacterial 16S rRNA gene sequences were amplified and sequenced. Analysis allowed for taxonomic identification, and quantitative PCR was used to quantify the bacterial load within samples. RESULTS: NEC samples generally contained an increased total burden of bacteria. NEC and non-NEC sample sets were both marked by high inter-individual variability and an abundance of opportunistic pathogens. There was no statistically significant distinction between the composition of NEC and non-NEC microbial communities. K-means clustering enabled us to identify several stable clusters, including clusters of NEC and midgut volvulus samples enriched with Clostridium and Bacteroides. Another cluster containing both NEC and non-NEC samples was marked by an abundance of Enterobacteriaceae and decreased diversity among NEC samples. CONCLUSIONS: The results indicate that NEC is a disease without a uniform pattern of microbial colonization, but that NEC is associated with an abundance of strict anaerobes and a decrease in community diversity. Public Library of Science 2014-09-09 /pmc/articles/PMC4159227/ /pubmed/25203729 http://dx.doi.org/10.1371/journal.pone.0105046 Text en © 2014 Brower-Sinning et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Brower-Sinning, Rachel
Zhong, Diana
Good, Misty
Firek, Brian
Baker, Robyn
Sodhi, Chhinder P.
Hackam, David J.
Morowitz, Michael J.
Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis
title Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis
title_full Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis
title_fullStr Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis
title_full_unstemmed Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis
title_short Mucosa-Associated Bacterial Diversity in Necrotizing Enterocolitis
title_sort mucosa-associated bacterial diversity in necrotizing enterocolitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159227/
https://www.ncbi.nlm.nih.gov/pubmed/25203729
http://dx.doi.org/10.1371/journal.pone.0105046
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