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Early changes of muscle membrane properties in porcine faecal peritonitis
INTRODUCTION: Sepsis-induced myopathy and critical illness myopathy (CIM) are possible causes of muscle weakness in intensive care patients. They have been attributed to muscle membrane dysfunction. The aim of this study was to investigate membrane properties in the early stage of experimental sepsi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159512/ https://www.ncbi.nlm.nih.gov/pubmed/25145497 http://dx.doi.org/10.1186/s13054-014-0484-2 |
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author | Ackermann, Karin A Bostock, Hugh Brander, Lukas Schröder, Ralph Djafarzadeh, Siamak Tuchscherer, Daniel Jakob, Stephan M Takala, Jukka Z’Graggen, Werner J |
author_facet | Ackermann, Karin A Bostock, Hugh Brander, Lukas Schröder, Ralph Djafarzadeh, Siamak Tuchscherer, Daniel Jakob, Stephan M Takala, Jukka Z’Graggen, Werner J |
author_sort | Ackermann, Karin A |
collection | PubMed |
description | INTRODUCTION: Sepsis-induced myopathy and critical illness myopathy (CIM) are possible causes of muscle weakness in intensive care patients. They have been attributed to muscle membrane dysfunction. The aim of this study was to investigate membrane properties in the early stage of experimental sepsis by evaluating muscle excitability. METHODS: In total, 20 anaesthetized and mechanically ventilated pigs were randomized to either faecal peritonitis (n = 10) or to non-septic controls (n = 10). Resuscitation with fluids and vasoactive drugs was started 3 hours after peritonitis induction. Muscle membrane properties were investigated by measuring muscle velocity recovery cycles before induction of peritonitis as well as 6, 18 and 27 hours thereafter. Muscle relative refractory period (MRRP) and early supernormality (ESN) were assessed. RESULTS: Peritonitis lasting 27 hours was associated with an increase of MRRP by 28% from 2.38 ± 0.18 ms (mean ± SD) to 3.47 ± 1.79 ms (P <0.01) and a decrease of ESN by 31% from 9.64 ± 2.82% to 6.50 ± 2.64% (P <0.01). ESN reduction was already apparent 6 hours after induction of peritonitis. Values in controls did not show any significant alterations. CONCLUSIONS: Muscle membrane abnormalities consistent with membrane depolarization and/or sodium channel inactivation occurred within 6 hours of peritonitis induction. This indicates that changes that have been described in established sepsis-induced myopathy and/or CIM start early in the course of sepsis. Muscle excitability testing facilitates evaluation of the time course of these changes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13054-014-0484-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4159512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41595122014-09-11 Early changes of muscle membrane properties in porcine faecal peritonitis Ackermann, Karin A Bostock, Hugh Brander, Lukas Schröder, Ralph Djafarzadeh, Siamak Tuchscherer, Daniel Jakob, Stephan M Takala, Jukka Z’Graggen, Werner J Crit Care Research INTRODUCTION: Sepsis-induced myopathy and critical illness myopathy (CIM) are possible causes of muscle weakness in intensive care patients. They have been attributed to muscle membrane dysfunction. The aim of this study was to investigate membrane properties in the early stage of experimental sepsis by evaluating muscle excitability. METHODS: In total, 20 anaesthetized and mechanically ventilated pigs were randomized to either faecal peritonitis (n = 10) or to non-septic controls (n = 10). Resuscitation with fluids and vasoactive drugs was started 3 hours after peritonitis induction. Muscle membrane properties were investigated by measuring muscle velocity recovery cycles before induction of peritonitis as well as 6, 18 and 27 hours thereafter. Muscle relative refractory period (MRRP) and early supernormality (ESN) were assessed. RESULTS: Peritonitis lasting 27 hours was associated with an increase of MRRP by 28% from 2.38 ± 0.18 ms (mean ± SD) to 3.47 ± 1.79 ms (P <0.01) and a decrease of ESN by 31% from 9.64 ± 2.82% to 6.50 ± 2.64% (P <0.01). ESN reduction was already apparent 6 hours after induction of peritonitis. Values in controls did not show any significant alterations. CONCLUSIONS: Muscle membrane abnormalities consistent with membrane depolarization and/or sodium channel inactivation occurred within 6 hours of peritonitis induction. This indicates that changes that have been described in established sepsis-induced myopathy and/or CIM start early in the course of sepsis. Muscle excitability testing facilitates evaluation of the time course of these changes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13054-014-0484-2) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-22 2014 /pmc/articles/PMC4159512/ /pubmed/25145497 http://dx.doi.org/10.1186/s13054-014-0484-2 Text en © Ackermann et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ackermann, Karin A Bostock, Hugh Brander, Lukas Schröder, Ralph Djafarzadeh, Siamak Tuchscherer, Daniel Jakob, Stephan M Takala, Jukka Z’Graggen, Werner J Early changes of muscle membrane properties in porcine faecal peritonitis |
title | Early changes of muscle membrane properties in porcine faecal peritonitis |
title_full | Early changes of muscle membrane properties in porcine faecal peritonitis |
title_fullStr | Early changes of muscle membrane properties in porcine faecal peritonitis |
title_full_unstemmed | Early changes of muscle membrane properties in porcine faecal peritonitis |
title_short | Early changes of muscle membrane properties in porcine faecal peritonitis |
title_sort | early changes of muscle membrane properties in porcine faecal peritonitis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159512/ https://www.ncbi.nlm.nih.gov/pubmed/25145497 http://dx.doi.org/10.1186/s13054-014-0484-2 |
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