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Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

BACKGROUND: Pichia pastoris is a widely-used host for recombinant protein production; expression is typically driven by methanol-inducible alcohol oxidase (AOX) promoters. Recently this system has become an important source of recombinant G protein-coupled receptors (GPCRs) for structural biology an...

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Detalles Bibliográficos
Autores principales:	Bawa, Zharain, Routledge, Sarah J, Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian, Ganzlin, Markus, Poyner, David R, Bill, Roslyn M
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159547/ https://www.ncbi.nlm.nih.gov/pubmed/25186468 http://dx.doi.org/10.1186/s12934-014-0127-y

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