Cargando…
The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice
B12D belongs to a function unknown subgroup of the Balem (Barley aleurone and embryo) proteins. In our previous work on rice seed germination, we identified a B12D-like protein encoded by LOC_Os7g41350 (named OsB12D1). OsB12D1 pertains to an ancient protein family with an amino acid sequence highly...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159805/ https://www.ncbi.nlm.nih.gov/pubmed/25089878 http://dx.doi.org/10.3390/ijms150813461 |
_version_ | 1782334282658742272 |
---|---|
author | He, Dongli Zhang, Hui Yang, Pingfang |
author_facet | He, Dongli Zhang, Hui Yang, Pingfang |
author_sort | He, Dongli |
collection | PubMed |
description | B12D belongs to a function unknown subgroup of the Balem (Barley aleurone and embryo) proteins. In our previous work on rice seed germination, we identified a B12D-like protein encoded by LOC_Os7g41350 (named OsB12D1). OsB12D1 pertains to an ancient protein family with an amino acid sequence highly conserved from moss to angiosperms. Among the six OsB12Ds, OsB12D1 is one of the major transcripts and is primarily expressed in germinating seed and root. Bioinformatics analyses indicated that OsB12D1 is an anoxic or submergence resistance-related gene. RT-PCR results showed OsB12D1 is induced remarkably in the coleoptiles or roots by flooding during seed germination and early seedling growth. The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type. Moreover, this tolerance was not affected by the gibberellin biosynthesis inhibitor paclobutrazol. OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions. This work indicated that OsB12D1 is a promising gene that can help to enhance rice seedling establishment in farming practices, especially for direct seeding. |
format | Online Article Text |
id | pubmed-4159805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-41598052014-09-18 The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice He, Dongli Zhang, Hui Yang, Pingfang Int J Mol Sci Article B12D belongs to a function unknown subgroup of the Balem (Barley aleurone and embryo) proteins. In our previous work on rice seed germination, we identified a B12D-like protein encoded by LOC_Os7g41350 (named OsB12D1). OsB12D1 pertains to an ancient protein family with an amino acid sequence highly conserved from moss to angiosperms. Among the six OsB12Ds, OsB12D1 is one of the major transcripts and is primarily expressed in germinating seed and root. Bioinformatics analyses indicated that OsB12D1 is an anoxic or submergence resistance-related gene. RT-PCR results showed OsB12D1 is induced remarkably in the coleoptiles or roots by flooding during seed germination and early seedling growth. The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type. Moreover, this tolerance was not affected by the gibberellin biosynthesis inhibitor paclobutrazol. OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions. This work indicated that OsB12D1 is a promising gene that can help to enhance rice seedling establishment in farming practices, especially for direct seeding. MDPI 2014-07-31 /pmc/articles/PMC4159805/ /pubmed/25089878 http://dx.doi.org/10.3390/ijms150813461 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article He, Dongli Zhang, Hui Yang, Pingfang The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice |
title | The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice |
title_full | The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice |
title_fullStr | The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice |
title_full_unstemmed | The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice |
title_short | The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice |
title_sort | mitochondrion-located protein osb12d1 enhances flooding tolerance during seed germination and early seedling growth in rice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159805/ https://www.ncbi.nlm.nih.gov/pubmed/25089878 http://dx.doi.org/10.3390/ijms150813461 |
work_keys_str_mv | AT hedongli themitochondrionlocatedproteinosb12d1enhancesfloodingtoleranceduringseedgerminationandearlyseedlinggrowthinrice AT zhanghui themitochondrionlocatedproteinosb12d1enhancesfloodingtoleranceduringseedgerminationandearlyseedlinggrowthinrice AT yangpingfang themitochondrionlocatedproteinosb12d1enhancesfloodingtoleranceduringseedgerminationandearlyseedlinggrowthinrice AT hedongli mitochondrionlocatedproteinosb12d1enhancesfloodingtoleranceduringseedgerminationandearlyseedlinggrowthinrice AT zhanghui mitochondrionlocatedproteinosb12d1enhancesfloodingtoleranceduringseedgerminationandearlyseedlinggrowthinrice AT yangpingfang mitochondrionlocatedproteinosb12d1enhancesfloodingtoleranceduringseedgerminationandearlyseedlinggrowthinrice |