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A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting

She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess...

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Autores principales: Di Girolamo, Francesco, Masotti, Andrea, Salvatori, Guglielmo, Scapaticci, Margherita, Muraca, Maurizio, Putignani, Lorenza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159820/
https://www.ncbi.nlm.nih.gov/pubmed/25110863
http://dx.doi.org/10.3390/ijms150813697
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author Di Girolamo, Francesco
Masotti, Andrea
Salvatori, Guglielmo
Scapaticci, Margherita
Muraca, Maurizio
Putignani, Lorenza
author_facet Di Girolamo, Francesco
Masotti, Andrea
Salvatori, Guglielmo
Scapaticci, Margherita
Muraca, Maurizio
Putignani, Lorenza
author_sort Di Girolamo, Francesco
collection PubMed
description She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures.
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spelling pubmed-41598202014-09-18 A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting Di Girolamo, Francesco Masotti, Andrea Salvatori, Guglielmo Scapaticci, Margherita Muraca, Maurizio Putignani, Lorenza Int J Mol Sci Article She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures. MDPI 2014-08-08 /pmc/articles/PMC4159820/ /pubmed/25110863 http://dx.doi.org/10.3390/ijms150813697 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Di Girolamo, Francesco
Masotti, Andrea
Salvatori, Guglielmo
Scapaticci, Margherita
Muraca, Maurizio
Putignani, Lorenza
A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
title A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
title_full A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
title_fullStr A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
title_full_unstemmed A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
title_short A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting
title_sort sensitive and effective proteomic approach to identify she-donkey’s and goat’s milk adulterations by maldi-tof ms fingerprinting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159820/
https://www.ncbi.nlm.nih.gov/pubmed/25110863
http://dx.doi.org/10.3390/ijms150813697
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