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Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1

To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines...

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Autores principales: Tuan, Pham Anh, Kwon, Do Yeon, Lee, Sanghyun, Arasu, Mariadhas Valan, Al-Dhabi, Naif Abdullah, Park, Nam Il, Park, Sang Un
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159879/
https://www.ncbi.nlm.nih.gov/pubmed/25153629
http://dx.doi.org/10.3390/ijms150814743
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author Tuan, Pham Anh
Kwon, Do Yeon
Lee, Sanghyun
Arasu, Mariadhas Valan
Al-Dhabi, Naif Abdullah
Park, Nam Il
Park, Sang Un
author_facet Tuan, Pham Anh
Kwon, Do Yeon
Lee, Sanghyun
Arasu, Mariadhas Valan
Al-Dhabi, Naif Abdullah
Park, Nam Il
Park, Sang Un
author_sort Tuan, Pham Anh
collection PubMed
description To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines showing over-expression of AtPAP1 were generated. In order to investigate the regulation of AtPAP1 on the activities of CGA biosynthetic genes, the expression levels of seven P. grandiflorum CGA biosynthetic genes were analyzed in the hairy root line that had the greatest accumulation of AtPAP1 transcript, OxPAP1-1. The introduction of AtPAP1 increased the mRNA levels of all examined CGA biosynthetic genes and resulted in a 900% up-regulation of CGA accumulation in OxPAP1-1 hairy roots relative to controls. This suggests that P. grandiflorum hairy roots that over-express the AtPAP1 gene are a potential alternative source of roots for the production of CGA.
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spelling pubmed-41598792014-09-18 Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1 Tuan, Pham Anh Kwon, Do Yeon Lee, Sanghyun Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Park, Nam Il Park, Sang Un Int J Mol Sci Article To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines showing over-expression of AtPAP1 were generated. In order to investigate the regulation of AtPAP1 on the activities of CGA biosynthetic genes, the expression levels of seven P. grandiflorum CGA biosynthetic genes were analyzed in the hairy root line that had the greatest accumulation of AtPAP1 transcript, OxPAP1-1. The introduction of AtPAP1 increased the mRNA levels of all examined CGA biosynthetic genes and resulted in a 900% up-regulation of CGA accumulation in OxPAP1-1 hairy roots relative to controls. This suggests that P. grandiflorum hairy roots that over-express the AtPAP1 gene are a potential alternative source of roots for the production of CGA. MDPI 2014-08-22 /pmc/articles/PMC4159879/ /pubmed/25153629 http://dx.doi.org/10.3390/ijms150814743 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Tuan, Pham Anh
Kwon, Do Yeon
Lee, Sanghyun
Arasu, Mariadhas Valan
Al-Dhabi, Naif Abdullah
Park, Nam Il
Park, Sang Un
Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
title Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
title_full Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
title_fullStr Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
title_full_unstemmed Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
title_short Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
title_sort enhancement of chlorogenic acid production in hairy roots of platycodon grandiflorum by over-expression of an arabidopsis thaliana transcription factor atpap1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159879/
https://www.ncbi.nlm.nih.gov/pubmed/25153629
http://dx.doi.org/10.3390/ijms150814743
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