Cargando…

Affinity Purification of Binding miRNAs for Messenger RNA Fused with a Common Tag

Prediction of microRNA–mRNA interaction typically relies on bioinformatic methods, but these methods only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. A major obstacle to the miRNA research has been the lack of experimental proced...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wei, Ke, Yan, Feng, Xiao, Hui, Yang, Xiaoxu, Xie, Guie, Xiao, Ye, Wang, Tingting, Xun, Yu, Huang, Zhaoqin, Han, Mei, Zhang, Jian, Xiang, Shuanglin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159880/ https://www.ncbi.nlm.nih.gov/pubmed/25153630 http://dx.doi.org/10.3390/ijms150814753

Ejemplares similares

Purification and Identification of miRNA Target Sites in Genome Using DNA Affinity Precipitation
por: Xun, Yu, et al.
Publicado: (2019)

Corrigendum: Purification and Identification of miRNA Target Sites in Genome Using DNA Affinity Precipitation
por: Xun, Yu, et al.
Publicado: (2020)

Several Affinity Tags Commonly Used in Chromatographic Purification
por: Zhao, Xinyu, et al.
Publicado: (2013)

Purification of Messenger Ribonucleoprotein Particles via a Tagged Nascent Polypeptide
por: Inchaustegui Gil, Diana P., et al.
Publicado: (2016)

Tandem affinity purification of miRNA target mRNAs (TAP-Tar)
por: Nonne, Nora, et al.
Publicado: (2010)

High-throughput Purification of Affinity-tagged Recombinant Proteins
por: Wiesler, Simone C., et al.
Publicado: (2012)

The ARiBo tag: a reliable tool for affinity purification of RNAs under native conditions
por: Di Tomasso, Geneviève, et al.
Publicado: (2011)

Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis
por: Schoonen, Lise, et al.
Publicado: (2017)

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
por: Nilvebrant, Johan, et al.
Publicado: (2012)

Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells
por: Ti, Shih-Chieh, et al.
Publicado: (2020)

Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)
por: Braun, Juliane, et al.
Publicado: (2014)

Identification of sitagliptin binding proteins by affinity purification mass spectrometry: Sitagliptin binding proteins identified by affinity purification mass spectrometry
por: Wang, Xue-Ning, et al.
Publicado: (2022)

SnAvi – a new tandem tag for high-affinity protein-complex purification
por: Schäffer, Ursula, et al.
Publicado: (2010)

In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins
por: Buhrman, Jason S, et al.
Publicado: (2012)

Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica
por: Rauwolf, Stefan, et al.
Publicado: (2021)

Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins
por: Sawin, Kenneth E., et al.
Publicado: (2010)

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
por: Scheich, Christoph, et al.
Publicado: (2003)

Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium
por: Meima, Marcel E., et al.
Publicado: (2007)

Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification
por: Zhang, Shuang, et al.
Publicado: (2010)

Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags
por: Salvail-Lacoste, Alix, et al.
Publicado: (2013)

A Chimeric Affinity Tag for Efficient Expression and Chromatographic Purification of Heterologous Proteins from Plants
por: Sainsbury, Frank, et al.
Publicado: (2016)

One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles
por: Zhou, Li‐Jian, et al.
Publicado: (2021)

Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis
por: Tani, Naoki, et al.
Publicado: (2022)

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
por: Kim, Ji Hun, et al.
Publicado: (2010)

Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling
por: Fogen, Dawson, et al.
Publicado: (2015)

Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs
por: Mickutė, Milda, et al.
Publicado: (2021)

Identification of potential micro-messenger RNAs (miRNA–mRNA) interaction network of osteosarcoma
por: Xu, Keteng, et al.
Publicado: (2021)

Sequential affinity purification of peroxidase tagged bispecific anti-SARS-CoV antibodies on phenylboronic acid agarose
por: Bhatnagar, Pravin K., et al.
Publicado: (2008)

Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer
por: Kilisch, Markus, et al.
Publicado: (2021)

Non-Affinity Purification of Antibodies
por: Arakawa, Tsutomu, et al.
Publicado: (2023)

Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila
por: Yilmaz, Gürkan, et al.
Publicado: (2015)

Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body
por: Minkner, Robert, et al.
Publicado: (2020)

Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1
por: Zhou, Jie, et al.
Publicado: (2020)

Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli, Purification, and Characterization
por: Cserjan-Puschmann, Monika, et al.
Publicado: (2020)

Posttranscriptional modification of messenger RNAs in eukaryotes
por: Laptev, I. G., et al.
Publicado: (2015)

Profiling of Parkin-Binding Partners Using Tandem Affinity Purification
por: Zanon, Alessandra, et al.
Publicado: (2013)

Falling water ice affinity purification of ice-binding proteins
por: Adar, Chen, et al.
Publicado: (2018)

Heparin affinity purification of extracellular vesicles
por: Balaj, Leonora, et al.
Publicado: (2015)

Affinity Membranes and Monoliths for Protein Purification
por: Lalli, Eleonora, et al.
Publicado: (2019)

MAP Tag: A Novel Tagging System for Protein Purification and Detection
por: Fujii, Yuki, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_j4e2esl9bnl2g0475a6ivg6ld3