Antitumor effects of dammarane-type saponins from steamed Notoginseng

Six dammarane-type saponins were extracted from steamed Panax notoginseng. Their chemical structures were identified spectroscopically as ginsenosides Rh(1) (1), Rg(1) (2), 20 (S)-Rg(3) (3), 20 (R)-Rg(3) (4), Rb(3) (5), and Rb(1) (6). Compounds (0.1-10 μM) were tested for inhibition of tumor necrosi...

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Detalles Bibliográficos
Autores principales: He, Fan, Ding, Yan, Liang, Chun, Song, Seok Bean, Dou, De-Qiang, Song, Gyu Yong, Kim, Young Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159925/
https://www.ncbi.nlm.nih.gov/pubmed/25210319
http://dx.doi.org/10.4103/0973-1296.137372
Descripción
Sumario:Six dammarane-type saponins were extracted from steamed Panax notoginseng. Their chemical structures were identified spectroscopically as ginsenosides Rh(1) (1), Rg(1) (2), 20 (S)-Rg(3) (3), 20 (R)-Rg(3) (4), Rb(3) (5), and Rb(1) (6). Compounds (0.1-10 μM) were tested for inhibition of tumor necrosis factor-α (TNF)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) luciferase reporter activity using a human kidney 293T cell-based assay. Ginsenoside Rb(3) (5) showed the most significant activity with an IC(50) of 8.2 μM. This compound also inhibited the induction of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) messenger Ribonucleic acid (mRNA) in a dose-dependent manner after HepG2 cells had been treated with TNF-α (10 ng/mL).

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