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A quantitative fluorescence‐based steady‐state assay of DNA polymerase
Fluorescent dyes that bind DNA have been demonstrated as a useful alternative to radionucleotides for the quantification of DNA and the in vitro measurement of the activity of DNA polymerases and nucleases. However, this approach is generally used in a semi‐quantitative way to determine relative rat...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160019/ https://www.ncbi.nlm.nih.gov/pubmed/24860875 http://dx.doi.org/10.1111/febs.12760 |