Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients

Osteoarthritis (OA) is considered the most prevalent form of arthritis. The aim of this study was to verify potential protein OA biomarkers by applying Selected Reaction Monitoring (SRM) assays to protein extracts obtained from Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) isolated from OA patients....

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Autores principales: Camafeita, Emilio, Lamas, José-Ramón, Calvo, Enrique, Tornero-Esteban, Pilar, López, Juan-Antonio, Fernández-Gutiérrez, Benjamín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160139/
https://www.ncbi.nlm.nih.gov/pubmed/25249828
http://dx.doi.org/10.1186/1559-0275-11-33
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author Camafeita, Emilio
Lamas, José-Ramón
Calvo, Enrique
Tornero-Esteban, Pilar
López, Juan-Antonio
Fernández-Gutiérrez, Benjamín
author_facet Camafeita, Emilio
Lamas, José-Ramón
Calvo, Enrique
Tornero-Esteban, Pilar
López, Juan-Antonio
Fernández-Gutiérrez, Benjamín
author_sort Camafeita, Emilio
collection PubMed
description Osteoarthritis (OA) is considered the most prevalent form of arthritis. The aim of this study was to verify potential protein OA biomarkers by applying Selected Reaction Monitoring (SRM) assays to protein extracts obtained from Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) isolated from OA patients. BM aspirates were obtained from the femoral channel of OA patients at the time of surgery and from the femoral channel of hip fracture subjects without OA during hip joint replacement surgery for the treatment of subcapital fracture. SRM results verified the differential expression of several protein biomarkers in BM-MSCs from OA patients.
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spelling pubmed-41601392014-09-23 Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients Camafeita, Emilio Lamas, José-Ramón Calvo, Enrique Tornero-Esteban, Pilar López, Juan-Antonio Fernández-Gutiérrez, Benjamín Clin Proteomics Letter to the Editor Osteoarthritis (OA) is considered the most prevalent form of arthritis. The aim of this study was to verify potential protein OA biomarkers by applying Selected Reaction Monitoring (SRM) assays to protein extracts obtained from Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) isolated from OA patients. BM aspirates were obtained from the femoral channel of OA patients at the time of surgery and from the femoral channel of hip fracture subjects without OA during hip joint replacement surgery for the treatment of subcapital fracture. SRM results verified the differential expression of several protein biomarkers in BM-MSCs from OA patients. Springer 2014-09-01 /pmc/articles/PMC4160139/ /pubmed/25249828 http://dx.doi.org/10.1186/1559-0275-11-33 Text en Copyright © 2014 Camafeita et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Camafeita, Emilio
Lamas, José-Ramón
Calvo, Enrique
Tornero-Esteban, Pilar
López, Juan-Antonio
Fernández-Gutiérrez, Benjamín
Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
title Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
title_full Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
title_fullStr Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
title_full_unstemmed Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
title_short Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
title_sort selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160139/
https://www.ncbi.nlm.nih.gov/pubmed/25249828
http://dx.doi.org/10.1186/1559-0275-11-33
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