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De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée)
BACKGROUND: Orthopteran migratory locust, Locusta migratoria, and lepidopteran Asian corn borer, Ostrinia furnacalis, are two types of insects undergoing incomplete and complete metamorphosis, respectively. Identification of candidate genes regulating wing development in these two insects would prov...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160219/ https://www.ncbi.nlm.nih.gov/pubmed/25207539 http://dx.doi.org/10.1371/journal.pone.0106770 |
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author | Liu, Suning Wei, Wei Chu, Yuan Zhang, Long Shen, Jie An, Chunju |
author_facet | Liu, Suning Wei, Wei Chu, Yuan Zhang, Long Shen, Jie An, Chunju |
author_sort | Liu, Suning |
collection | PubMed |
description | BACKGROUND: Orthopteran migratory locust, Locusta migratoria, and lepidopteran Asian corn borer, Ostrinia furnacalis, are two types of insects undergoing incomplete and complete metamorphosis, respectively. Identification of candidate genes regulating wing development in these two insects would provide insights into the further study about the molecular mechanisms controlling metamorphosis development. We have sequenced the transcriptome of O. furnacalis larvae previously. Here we sequenced and characterized the transcriptome of L. migratoria wing discs with special emphasis on wing development-related signaling pathways. METHODOLOGY/PRINCIPAL FINDINGS: Illumina Hiseq2000 was used to sequence 8.38 Gb of the transcriptome from dissected nymphal wing discs. De novo assembly generated 91,907 unigenes with mean length of 610 nt. All unigenes were searched against five databases including Nt, Nr, Swiss-Prot, COG, and KEGG for annotations using blastn or blastx algorithm with an cut-off E-value of 10(−5). A total of 23,359 (25.4%) unigenes have homologs within at least one database. Based on sequence similarity to homologs known to regulate Drosophila melanogaster wing development, we identified 50 and 46 potential wing development-related unigenes from L. migratoria and O. furnacalis transcriptome, respectively. The identified unigenes encode putative orthologs for nearly all components of the Hedgehog (Hh), Decapentaplegic (Dpp), Notch (N), and Wingless (Wg) signaling pathways, which are essential for growth and pattern formation during wing development. We investigated the expression profiles of the component genes involved in these signaling pathways in forewings and hind wings of L. migratoria and O. furnacalis. The results revealed the tested genes had different expression patterns in two insects. CONCLUSIONS/SIGNIFICANCE: This study provides the comprehensive sequence resource of the wing development-related signaling pathways of L. migratoria. The obtained data gives an insight into better understanding the molecular mechanisms involved in the wing development in L. migratoria and O. furnacalis, two insect species with different metamorphosis types. |
format | Online Article Text |
id | pubmed-4160219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41602192014-09-12 De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) Liu, Suning Wei, Wei Chu, Yuan Zhang, Long Shen, Jie An, Chunju PLoS One Research Article BACKGROUND: Orthopteran migratory locust, Locusta migratoria, and lepidopteran Asian corn borer, Ostrinia furnacalis, are two types of insects undergoing incomplete and complete metamorphosis, respectively. Identification of candidate genes regulating wing development in these two insects would provide insights into the further study about the molecular mechanisms controlling metamorphosis development. We have sequenced the transcriptome of O. furnacalis larvae previously. Here we sequenced and characterized the transcriptome of L. migratoria wing discs with special emphasis on wing development-related signaling pathways. METHODOLOGY/PRINCIPAL FINDINGS: Illumina Hiseq2000 was used to sequence 8.38 Gb of the transcriptome from dissected nymphal wing discs. De novo assembly generated 91,907 unigenes with mean length of 610 nt. All unigenes were searched against five databases including Nt, Nr, Swiss-Prot, COG, and KEGG for annotations using blastn or blastx algorithm with an cut-off E-value of 10(−5). A total of 23,359 (25.4%) unigenes have homologs within at least one database. Based on sequence similarity to homologs known to regulate Drosophila melanogaster wing development, we identified 50 and 46 potential wing development-related unigenes from L. migratoria and O. furnacalis transcriptome, respectively. The identified unigenes encode putative orthologs for nearly all components of the Hedgehog (Hh), Decapentaplegic (Dpp), Notch (N), and Wingless (Wg) signaling pathways, which are essential for growth and pattern formation during wing development. We investigated the expression profiles of the component genes involved in these signaling pathways in forewings and hind wings of L. migratoria and O. furnacalis. The results revealed the tested genes had different expression patterns in two insects. CONCLUSIONS/SIGNIFICANCE: This study provides the comprehensive sequence resource of the wing development-related signaling pathways of L. migratoria. The obtained data gives an insight into better understanding the molecular mechanisms involved in the wing development in L. migratoria and O. furnacalis, two insect species with different metamorphosis types. Public Library of Science 2014-09-10 /pmc/articles/PMC4160219/ /pubmed/25207539 http://dx.doi.org/10.1371/journal.pone.0106770 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Suning Wei, Wei Chu, Yuan Zhang, Long Shen, Jie An, Chunju De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) |
title |
De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) |
title_full |
De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) |
title_fullStr |
De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) |
title_full_unstemmed |
De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) |
title_short |
De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée) |
title_sort | de novo transcriptome analysis of wing development-related signaling pathways in locusta migratoria manilensis and ostrinia furnacalis (guenée) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160219/ https://www.ncbi.nlm.nih.gov/pubmed/25207539 http://dx.doi.org/10.1371/journal.pone.0106770 |
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