Cargando…

Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile

We investigated the effects of hepatic ischemia/reperfusion (I/R) injury on asymmetric dimethylarginine (ADMA, a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (involved, resp., in ADMA synthesis and degradation), and the cationi...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferrigno, Andrea, Rizzo, Vittoria, Bianchi, Alberto, Di Pasqua, Laura G., Berardo, Clarissa, Richelmi, Plinio, Vairetti, Mariapia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160639/
https://www.ncbi.nlm.nih.gov/pubmed/25243167
http://dx.doi.org/10.1155/2014/627434
_version_ 1782334426853670912
author Ferrigno, Andrea
Rizzo, Vittoria
Bianchi, Alberto
Di Pasqua, Laura G.
Berardo, Clarissa
Richelmi, Plinio
Vairetti, Mariapia
author_facet Ferrigno, Andrea
Rizzo, Vittoria
Bianchi, Alberto
Di Pasqua, Laura G.
Berardo, Clarissa
Richelmi, Plinio
Vairetti, Mariapia
author_sort Ferrigno, Andrea
collection PubMed
description We investigated the effects of hepatic ischemia/reperfusion (I/R) injury on asymmetric dimethylarginine (ADMA, a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (involved, resp., in ADMA synthesis and degradation), and the cationic transporter (CAT). Male Wistar rats were subjected to 30 or 60 min hepatic ischemia followed by 60 min reperfusion. ADMA levels in serum and bile were determined. Tissue ADMA, DDAH activity, DDAH-1 and CAT-2 protein, DDAH-1 and PRMT-1 mRNA expression, GSH/GSSG, ROS production, and lipid peroxidation were detected. ADMA was found in bile. I/R increased serum and bile ADMA levels while an intracellular decrease was detected after 60 min ischemia. Decreased DDAH activity, mRNA, and protein expression were observed at the end of reperfusion. No significant difference was observed in GSH/GSSG, ROS, lipid peroxidation, and CAT-2; a decrease in PRMT-1 mRNA expression was found after I/R. Liver is responsible for the biliary excretion of ADMA, as documented here for the first time, and I/R injury is associated with an oxidative stress-independent alteration in DDAH activity. These data are a step forward in the understanding of the pathways that regulate serum, tissue, and biliary levels of ADMA in which DDAH enzyme plays a crucial role.
format Online
Article
Text
id pubmed-4160639
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-41606392014-09-21 Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile Ferrigno, Andrea Rizzo, Vittoria Bianchi, Alberto Di Pasqua, Laura G. Berardo, Clarissa Richelmi, Plinio Vairetti, Mariapia Biomed Res Int Research Article We investigated the effects of hepatic ischemia/reperfusion (I/R) injury on asymmetric dimethylarginine (ADMA, a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (involved, resp., in ADMA synthesis and degradation), and the cationic transporter (CAT). Male Wistar rats were subjected to 30 or 60 min hepatic ischemia followed by 60 min reperfusion. ADMA levels in serum and bile were determined. Tissue ADMA, DDAH activity, DDAH-1 and CAT-2 protein, DDAH-1 and PRMT-1 mRNA expression, GSH/GSSG, ROS production, and lipid peroxidation were detected. ADMA was found in bile. I/R increased serum and bile ADMA levels while an intracellular decrease was detected after 60 min ischemia. Decreased DDAH activity, mRNA, and protein expression were observed at the end of reperfusion. No significant difference was observed in GSH/GSSG, ROS, lipid peroxidation, and CAT-2; a decrease in PRMT-1 mRNA expression was found after I/R. Liver is responsible for the biliary excretion of ADMA, as documented here for the first time, and I/R injury is associated with an oxidative stress-independent alteration in DDAH activity. These data are a step forward in the understanding of the pathways that regulate serum, tissue, and biliary levels of ADMA in which DDAH enzyme plays a crucial role. Hindawi Publishing Corporation 2014 2014-08-27 /pmc/articles/PMC4160639/ /pubmed/25243167 http://dx.doi.org/10.1155/2014/627434 Text en Copyright © 2014 Andrea Ferrigno et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ferrigno, Andrea
Rizzo, Vittoria
Bianchi, Alberto
Di Pasqua, Laura G.
Berardo, Clarissa
Richelmi, Plinio
Vairetti, Mariapia
Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile
title Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile
title_full Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile
title_fullStr Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile
title_full_unstemmed Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile
title_short Changes in ADMA/DDAH Pathway after Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Bile
title_sort changes in adma/ddah pathway after hepatic ischemia/reperfusion injury in rats: the role of bile
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160639/
https://www.ncbi.nlm.nih.gov/pubmed/25243167
http://dx.doi.org/10.1155/2014/627434
work_keys_str_mv AT ferrignoandrea changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile
AT rizzovittoria changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile
AT bianchialberto changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile
AT dipasqualaurag changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile
AT berardoclarissa changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile
AT richelmiplinio changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile
AT vairettimariapia changesinadmaddahpathwayafterhepaticischemiareperfusioninjuryinratstheroleofbile