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Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)

Recent studies have demonstrated that Notch-1 expression is increased in the hippocampus of Alzheimer's disease patients. We speculate that Notch-1 signaling may be involved in PC12 cell apoptosis induced by amyloid beta-peptide (25–35) (Aβ(25–35)). In the present study, PC12 cells were culture...

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Autores principales: Liang, Huimin, Zhang, Yaozhou, Shi, Xiaoyan, Wei, Tianxiang, Lou, Jiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160856/
https://www.ncbi.nlm.nih.gov/pubmed/25221582
http://dx.doi.org/10.4103/1673-5374.137577
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author Liang, Huimin
Zhang, Yaozhou
Shi, Xiaoyan
Wei, Tianxiang
Lou, Jiyu
author_facet Liang, Huimin
Zhang, Yaozhou
Shi, Xiaoyan
Wei, Tianxiang
Lou, Jiyu
author_sort Liang, Huimin
collection PubMed
description Recent studies have demonstrated that Notch-1 expression is increased in the hippocampus of Alzheimer's disease patients. We speculate that Notch-1 signaling may be involved in PC12 cell apoptosis induced by amyloid beta-peptide (25–35) (Aβ(25–35)). In the present study, PC12 cells were cultured with different doses (0, 0.1, 1.0, 10 and 100 nmol/L) of N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester, a Notch-1 signaling pathway inhibitor, for 30 minutes. Then cultured cells were induced with Aβ(25–35) for 48 hours. Pretreatment of PC12 cells with high doses of N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (> 10 nmol/L) prolonged the survival of PC12 cells after Aβ(25–35) induction, decreased the expression of apoptosis-related proteins caspase-3, -8, -9, increased the activity of oxidative stress-related superoxide dismutase and catalase, inhibited the production of active oxygen, and reduced nuclear factor kappa B expression. This study indicates that the Notch-1 signaling pathway plays a pivotal role in Aβ(25–35)-induced PC12 apoptosis.
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spelling pubmed-41608562014-09-14 Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35) Liang, Huimin Zhang, Yaozhou Shi, Xiaoyan Wei, Tianxiang Lou, Jiyu Neural Regen Res Technical Updates Recent studies have demonstrated that Notch-1 expression is increased in the hippocampus of Alzheimer's disease patients. We speculate that Notch-1 signaling may be involved in PC12 cell apoptosis induced by amyloid beta-peptide (25–35) (Aβ(25–35)). In the present study, PC12 cells were cultured with different doses (0, 0.1, 1.0, 10 and 100 nmol/L) of N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester, a Notch-1 signaling pathway inhibitor, for 30 minutes. Then cultured cells were induced with Aβ(25–35) for 48 hours. Pretreatment of PC12 cells with high doses of N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (> 10 nmol/L) prolonged the survival of PC12 cells after Aβ(25–35) induction, decreased the expression of apoptosis-related proteins caspase-3, -8, -9, increased the activity of oxidative stress-related superoxide dismutase and catalase, inhibited the production of active oxygen, and reduced nuclear factor kappa B expression. This study indicates that the Notch-1 signaling pathway plays a pivotal role in Aβ(25–35)-induced PC12 apoptosis. Medknow Publications & Media Pvt Ltd 2014-07-01 /pmc/articles/PMC4160856/ /pubmed/25221582 http://dx.doi.org/10.4103/1673-5374.137577 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Updates
Liang, Huimin
Zhang, Yaozhou
Shi, Xiaoyan
Wei, Tianxiang
Lou, Jiyu
Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)
title Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)
title_full Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)
title_fullStr Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)
title_full_unstemmed Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)
title_short Role of Notch-1 signaling pathway in PC12 cell apoptosis induced by amyloid beta-peptide (25–35)
title_sort role of notch-1 signaling pathway in pc12 cell apoptosis induced by amyloid beta-peptide (25–35)
topic Technical Updates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160856/
https://www.ncbi.nlm.nih.gov/pubmed/25221582
http://dx.doi.org/10.4103/1673-5374.137577
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