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A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160926/ https://www.ncbi.nlm.nih.gov/pubmed/24521863 http://dx.doi.org/10.1538/expanim.63.55 |
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author | Higuchi, Yuichiro Kawai, Kenji Yamamoto, Masahumi Kuronuma, Miyuki Ando, Yasuhiko Katano, Ikumi Nakamura, Masato Suemizu, Hiroshi |
author_facet | Higuchi, Yuichiro Kawai, Kenji Yamamoto, Masahumi Kuronuma, Miyuki Ando, Yasuhiko Katano, Ikumi Nakamura, Masato Suemizu, Hiroshi |
author_sort | Higuchi, Yuichiro |
collection | PubMed |
description | The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation. |
format | Online Article Text |
id | pubmed-4160926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41609262014-10-21 A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues Higuchi, Yuichiro Kawai, Kenji Yamamoto, Masahumi Kuronuma, Miyuki Ando, Yasuhiko Katano, Ikumi Nakamura, Masato Suemizu, Hiroshi Exp Anim Original The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation. Japanese Association for Laboratory Animal Science 2014-02-07 2014 /pmc/articles/PMC4160926/ /pubmed/24521863 http://dx.doi.org/10.1538/expanim.63.55 Text en ©2014 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Higuchi, Yuichiro Kawai, Kenji Yamamoto, Masahumi Kuronuma, Miyuki Ando, Yasuhiko Katano, Ikumi Nakamura, Masato Suemizu, Hiroshi A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues |
title | A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for
Analyzingthe Microenvironment of Xenograft Tissues |
title_full | A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for
Analyzingthe Microenvironment of Xenograft Tissues |
title_fullStr | A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for
Analyzingthe Microenvironment of Xenograft Tissues |
title_full_unstemmed | A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for
Analyzingthe Microenvironment of Xenograft Tissues |
title_short | A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for
Analyzingthe Microenvironment of Xenograft Tissues |
title_sort | novel enhanced green fluorescentprotein-expressing nog mouse for
analyzingthe microenvironment of xenograft tissues |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160926/ https://www.ncbi.nlm.nih.gov/pubmed/24521863 http://dx.doi.org/10.1538/expanim.63.55 |
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