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A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues

The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which...

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Autores principales: Higuchi, Yuichiro, Kawai, Kenji, Yamamoto, Masahumi, Kuronuma, Miyuki, Ando, Yasuhiko, Katano, Ikumi, Nakamura, Masato, Suemizu, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160926/
https://www.ncbi.nlm.nih.gov/pubmed/24521863
http://dx.doi.org/10.1538/expanim.63.55
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author Higuchi, Yuichiro
Kawai, Kenji
Yamamoto, Masahumi
Kuronuma, Miyuki
Ando, Yasuhiko
Katano, Ikumi
Nakamura, Masato
Suemizu, Hiroshi
author_facet Higuchi, Yuichiro
Kawai, Kenji
Yamamoto, Masahumi
Kuronuma, Miyuki
Ando, Yasuhiko
Katano, Ikumi
Nakamura, Masato
Suemizu, Hiroshi
author_sort Higuchi, Yuichiro
collection PubMed
description The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation.
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spelling pubmed-41609262014-10-21 A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues Higuchi, Yuichiro Kawai, Kenji Yamamoto, Masahumi Kuronuma, Miyuki Ando, Yasuhiko Katano, Ikumi Nakamura, Masato Suemizu, Hiroshi Exp Anim Original The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation. Japanese Association for Laboratory Animal Science 2014-02-07 2014 /pmc/articles/PMC4160926/ /pubmed/24521863 http://dx.doi.org/10.1538/expanim.63.55 Text en ©2014 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Higuchi, Yuichiro
Kawai, Kenji
Yamamoto, Masahumi
Kuronuma, Miyuki
Ando, Yasuhiko
Katano, Ikumi
Nakamura, Masato
Suemizu, Hiroshi
A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
title A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
title_full A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
title_fullStr A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
title_full_unstemmed A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
title_short A Novel Enhanced Green FluorescentProtein-Expressing NOG Mouse for Analyzingthe Microenvironment of Xenograft Tissues
title_sort novel enhanced green fluorescentprotein-expressing nog mouse for analyzingthe microenvironment of xenograft tissues
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160926/
https://www.ncbi.nlm.nih.gov/pubmed/24521863
http://dx.doi.org/10.1538/expanim.63.55
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