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Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice

Adipose tissues are closely connected with the immune system. It has been suggested that metabolic syndromes such as type 2 diabetes, arteriosclerosis and liver steatosis can be attributed to adipose tissue inflammation characterized by macrophage infiltration. To understand a physiological and path...

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Autores principales: Kondo, Taisuke, Toyoshima, Yujiro, Ishii, Yoshiyuki, Kyuwa, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160955/
https://www.ncbi.nlm.nih.gov/pubmed/24172196
http://dx.doi.org/10.1538/expanim.62.319
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author Kondo, Taisuke
Toyoshima, Yujiro
Ishii, Yoshiyuki
Kyuwa, Shigeru
author_facet Kondo, Taisuke
Toyoshima, Yujiro
Ishii, Yoshiyuki
Kyuwa, Shigeru
author_sort Kondo, Taisuke
collection PubMed
description Adipose tissues are closely connected with the immune system. It has been suggested that metabolic syndromes such as type 2 diabetes, arteriosclerosis and liver steatosis can be attributed to adipose tissue inflammation characterized by macrophage infiltration. To understand a physiological and pathological role of natural killer T (NKT) cells on inflammation in adipose tissue, we characterized a subset of NKT cells in abdominal and subcutaneous adipose tissues in C57BL/6J mice fed normal or high-fat diets. NKT cells comprised a larger portion of lymphocytes in adipose tissues compared with the spleen and peripheral blood, with epididymal adipose tissue having the highest number of NKT cells. Furthermore, some NKT cells in adipose tissues expressed higher levels of CD69 and intracellular interferon-γ, whereas the Vβ repertoires of NKT cells in adipose tissues were similar to other cells. In obese mice fed a high-fat diet, adipose tissue inflammation had little effect on the Vβ repertoire of NKT cells in epididymal adipose tissues. We speculate that the NKT cells in adipose tissues may form an equivalent subset in other tissues and that these subsets are likely to participate in adipose tissue inflammation. Additionally, the high expression level of CD69 and intracellular IFN-γ raises the possibility that NKT cells in adipose tissue may be stimulated by some physiological mechanism.
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spelling pubmed-41609552014-10-21 Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice Kondo, Taisuke Toyoshima, Yujiro Ishii, Yoshiyuki Kyuwa, Shigeru Exp Anim Original Adipose tissues are closely connected with the immune system. It has been suggested that metabolic syndromes such as type 2 diabetes, arteriosclerosis and liver steatosis can be attributed to adipose tissue inflammation characterized by macrophage infiltration. To understand a physiological and pathological role of natural killer T (NKT) cells on inflammation in adipose tissue, we characterized a subset of NKT cells in abdominal and subcutaneous adipose tissues in C57BL/6J mice fed normal or high-fat diets. NKT cells comprised a larger portion of lymphocytes in adipose tissues compared with the spleen and peripheral blood, with epididymal adipose tissue having the highest number of NKT cells. Furthermore, some NKT cells in adipose tissues expressed higher levels of CD69 and intracellular interferon-γ, whereas the Vβ repertoires of NKT cells in adipose tissues were similar to other cells. In obese mice fed a high-fat diet, adipose tissue inflammation had little effect on the Vβ repertoire of NKT cells in epididymal adipose tissues. We speculate that the NKT cells in adipose tissues may form an equivalent subset in other tissues and that these subsets are likely to participate in adipose tissue inflammation. Additionally, the high expression level of CD69 and intracellular IFN-γ raises the possibility that NKT cells in adipose tissue may be stimulated by some physiological mechanism. Japanese Association for Laboratory Animal Science 2013-10-31 2013 /pmc/articles/PMC4160955/ /pubmed/24172196 http://dx.doi.org/10.1538/expanim.62.319 Text en ©2013 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Kondo, Taisuke
Toyoshima, Yujiro
Ishii, Yoshiyuki
Kyuwa, Shigeru
Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice
title Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice
title_full Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice
title_fullStr Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice
title_full_unstemmed Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice
title_short Natural Killer T Cells in Adipose Tissue Are Activated in Lean Mice
title_sort natural killer t cells in adipose tissue are activated in lean mice
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160955/
https://www.ncbi.nlm.nih.gov/pubmed/24172196
http://dx.doi.org/10.1538/expanim.62.319
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