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Mycobacterium Tuberculosis Infection in Rhesus Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10 as Immunodiagnostic Antigens(#)
Tuberculosis (TB) in nonhuman primates is a serious menace to the welfare of the animals and human who come into contact with them, while the rapid, accurate, and robust diagnosis is challenging. In this study, we first sought to establish an appropriate primate TB model resembling natural TB in non...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160956/ https://www.ncbi.nlm.nih.gov/pubmed/24172192 http://dx.doi.org/10.1538/expanim.62.281 |
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author | Min, Fangui Zhang, Yu Pan, Jinchun Wang, Jing Yuan, Wen |
author_facet | Min, Fangui Zhang, Yu Pan, Jinchun Wang, Jing Yuan, Wen |
author_sort | Min, Fangui |
collection | PubMed |
description | Tuberculosis (TB) in nonhuman primates is a serious menace to the welfare of the animals and human who come into contact with them, while the rapid, accurate, and robust diagnosis is challenging. In this study, we first sought to establish an appropriate primate TB model resembling natural TB in nonhuman primates. Four rhesus monkeys (Macaca mulatta) of Chinese origin were infected intratracheally with two low doses of M. tuberculosis H37Rv. Regardless of the infectious doses, all monkeys were demonstrated to be successfully infected by clinical assessments, tuberculin skin test conversions, peripheral immune responses, gross observations, histopathology analysis, and M. tuberculosis burdens. Furthermore, we extended the usefulness of this model for assessing the following immunodiagnostic antigens: CFP10, ESAT-6, CFP10-ESAT-6, and an antigen cocktail of CFP10 and ESAT-6. The data showed that CFP10 was an M. tuberculosis-specific, “early” antigen used for serodiagnosis of TB in nonhuman primates. In conclusion, we established a useful primate TB model depending on low doses of M .tuberculosis and affording new opportunities for studies of M. tuberculosis disease and diagnostics. |
format | Online Article Text |
id | pubmed-4160956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41609562014-10-21 Mycobacterium Tuberculosis Infection in Rhesus Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10 as Immunodiagnostic Antigens(#) Min, Fangui Zhang, Yu Pan, Jinchun Wang, Jing Yuan, Wen Exp Anim Original Tuberculosis (TB) in nonhuman primates is a serious menace to the welfare of the animals and human who come into contact with them, while the rapid, accurate, and robust diagnosis is challenging. In this study, we first sought to establish an appropriate primate TB model resembling natural TB in nonhuman primates. Four rhesus monkeys (Macaca mulatta) of Chinese origin were infected intratracheally with two low doses of M. tuberculosis H37Rv. Regardless of the infectious doses, all monkeys were demonstrated to be successfully infected by clinical assessments, tuberculin skin test conversions, peripheral immune responses, gross observations, histopathology analysis, and M. tuberculosis burdens. Furthermore, we extended the usefulness of this model for assessing the following immunodiagnostic antigens: CFP10, ESAT-6, CFP10-ESAT-6, and an antigen cocktail of CFP10 and ESAT-6. The data showed that CFP10 was an M. tuberculosis-specific, “early” antigen used for serodiagnosis of TB in nonhuman primates. In conclusion, we established a useful primate TB model depending on low doses of M .tuberculosis and affording new opportunities for studies of M. tuberculosis disease and diagnostics. Japanese Association for Laboratory Animal Science 2013-10-31 2013 /pmc/articles/PMC4160956/ /pubmed/24172192 http://dx.doi.org/10.1538/expanim.62.281 Text en ©2013 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Min, Fangui Zhang, Yu Pan, Jinchun Wang, Jing Yuan, Wen Mycobacterium Tuberculosis Infection in Rhesus Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10 as Immunodiagnostic Antigens(#) |
title | Mycobacterium Tuberculosis Infection in Rhesus
Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10
as Immunodiagnostic Antigens(#) |
title_full | Mycobacterium Tuberculosis Infection in Rhesus
Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10
as Immunodiagnostic Antigens(#) |
title_fullStr | Mycobacterium Tuberculosis Infection in Rhesus
Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10
as Immunodiagnostic Antigens(#) |
title_full_unstemmed | Mycobacterium Tuberculosis Infection in Rhesus
Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10
as Immunodiagnostic Antigens(#) |
title_short | Mycobacterium Tuberculosis Infection in Rhesus
Monkeys (Macaca mulatta) and Evaluation of ESAT-6 and CFP10
as Immunodiagnostic Antigens(#) |
title_sort | mycobacterium tuberculosis infection in rhesus
monkeys (macaca mulatta) and evaluation of esat-6 and cfp10
as immunodiagnostic antigens(#) |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160956/ https://www.ncbi.nlm.nih.gov/pubmed/24172192 http://dx.doi.org/10.1538/expanim.62.281 |
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