Cargando…
Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate Sodium-Induced Colitis
Ulcerative colitis (UC) is an inflammatory bowel disease, and its pathogenesis includes genetic, environmental, and immunological factors, such as T helper cells and their secreted cytokines. T helper cells are classified as Th1, Th2, and Th17 cells. However, it is unclear which T helper cells are i...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160978/ https://www.ncbi.nlm.nih.gov/pubmed/24770638 http://dx.doi.org/10.1538/expanim.63.133 |
_version_ | 1782334481515937792 |
---|---|
author | Okamura, Midori Yoh, Keigyou Ojima, Masami Morito, Naoki Takahashi, Satoru |
author_facet | Okamura, Midori Yoh, Keigyou Ojima, Masami Morito, Naoki Takahashi, Satoru |
author_sort | Okamura, Midori |
collection | PubMed |
description | Ulcerative colitis (UC) is an inflammatory bowel disease, and its pathogenesis includes genetic, environmental, and immunological factors, such as T helper cells and their secreted cytokines. T helper cells are classified as Th1, Th2, and Th17 cells. However, it is unclear which T helper cells are important in UC. Dextran sulfate sodium (DSS)-induced colitis is a commonly used model of UC. In this study, we induced DSS colitis in Th1 dominant (T-bet transgenic (Tg)) mice, Th2 dominant (GATA-3 Tg) mice, and Th17 dominant (RORγt Tg) mice to elucidate the roles of T helper cell in DSS colitis. The results showed that GATA-3 Tg mice developed the most severe DSS colitis compared with the other groups. GATA-3 Tg mice showed a significant decreased in weight from day 1 to day 7, and an increased high score for the disease activity index compared with the other groups. Furthermore, GATA-3 Tg mice developed many ulcers in the colon, and many neutrophils and macrophages were detected on day 4 after DSS treatment. Measurement of GATA-3-induced cytokines demonstrated that IL-13 was highly expressed in the colon from DSS-induced GATA-3 Tg mice. In conclusion, GATA-3 overexpression in T-cells and IL-13 might play important roles in the development of DSS colitis. |
format | Online Article Text |
id | pubmed-4160978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41609782014-10-21 Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate Sodium-Induced Colitis Okamura, Midori Yoh, Keigyou Ojima, Masami Morito, Naoki Takahashi, Satoru Exp Anim Original Ulcerative colitis (UC) is an inflammatory bowel disease, and its pathogenesis includes genetic, environmental, and immunological factors, such as T helper cells and their secreted cytokines. T helper cells are classified as Th1, Th2, and Th17 cells. However, it is unclear which T helper cells are important in UC. Dextran sulfate sodium (DSS)-induced colitis is a commonly used model of UC. In this study, we induced DSS colitis in Th1 dominant (T-bet transgenic (Tg)) mice, Th2 dominant (GATA-3 Tg) mice, and Th17 dominant (RORγt Tg) mice to elucidate the roles of T helper cell in DSS colitis. The results showed that GATA-3 Tg mice developed the most severe DSS colitis compared with the other groups. GATA-3 Tg mice showed a significant decreased in weight from day 1 to day 7, and an increased high score for the disease activity index compared with the other groups. Furthermore, GATA-3 Tg mice developed many ulcers in the colon, and many neutrophils and macrophages were detected on day 4 after DSS treatment. Measurement of GATA-3-induced cytokines demonstrated that IL-13 was highly expressed in the colon from DSS-induced GATA-3 Tg mice. In conclusion, GATA-3 overexpression in T-cells and IL-13 might play important roles in the development of DSS colitis. Japanese Association for Laboratory Animal Science 2014-04-26 2014 /pmc/articles/PMC4160978/ /pubmed/24770638 http://dx.doi.org/10.1538/expanim.63.133 Text en ©2014 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Okamura, Midori Yoh, Keigyou Ojima, Masami Morito, Naoki Takahashi, Satoru Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate Sodium-Induced Colitis |
title | Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate
Sodium-Induced Colitis |
title_full | Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate
Sodium-Induced Colitis |
title_fullStr | Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate
Sodium-Induced Colitis |
title_full_unstemmed | Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate
Sodium-Induced Colitis |
title_short | Overexpression of GATA-3 in T Cells Accelerates Dextran Sulfate
Sodium-Induced Colitis |
title_sort | overexpression of gata-3 in t cells accelerates dextran sulfate
sodium-induced colitis |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160978/ https://www.ncbi.nlm.nih.gov/pubmed/24770638 http://dx.doi.org/10.1538/expanim.63.133 |
work_keys_str_mv | AT okamuramidori overexpressionofgata3intcellsacceleratesdextransulfatesodiuminducedcolitis AT yohkeigyou overexpressionofgata3intcellsacceleratesdextransulfatesodiuminducedcolitis AT ojimamasami overexpressionofgata3intcellsacceleratesdextransulfatesodiuminducedcolitis AT moritonaoki overexpressionofgata3intcellsacceleratesdextransulfatesodiuminducedcolitis AT takahashisatoru overexpressionofgata3intcellsacceleratesdextransulfatesodiuminducedcolitis |