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Fast and sensitive multi-color 3D imaging using aberration-corrected multi-focus microscopy
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z-scanning and is often too slow to capture biological events. We report a new aberration-corrected multi-focus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifl...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161287/ https://www.ncbi.nlm.nih.gov/pubmed/23223154 http://dx.doi.org/10.1038/nmeth.2277 |
| _version_ | 1782334519810981888 |
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| author | Abrahamsson, Sara Chen, Jiji Hajj, Bassam Stallinga, Sjoerd Katsov, Alexander Y. Wisniewski, Jan Mizuguchi, Gaku Soulle, Pierre Mueller, Florian Darzacq, Claire Dugast Darzacq, Xavier Wu, Carl Bargmann, Cornelia I. Agard, David A. Dahan, Maxime Gustafsson, Mats G. L. |
| author_facet | Abrahamsson, Sara Chen, Jiji Hajj, Bassam Stallinga, Sjoerd Katsov, Alexander Y. Wisniewski, Jan Mizuguchi, Gaku Soulle, Pierre Mueller, Florian Darzacq, Claire Dugast Darzacq, Xavier Wu, Carl Bargmann, Cornelia I. Agard, David A. Dahan, Maxime Gustafsson, Mats G. L. |
| author_sort | Abrahamsson, Sara |
| collection | PubMed |
| description | Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z-scanning and is often too slow to capture biological events. We report a new aberration-corrected multi-focus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multi-focus system enables high-resolution 3D imaging in multiple colors with single molecule sensitivity, at speeds limited by the camera readout time of a single image. |
| format | Online Article Text |
| id | pubmed-4161287 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2012 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-41612872014-09-11 Fast and sensitive multi-color 3D imaging using aberration-corrected multi-focus microscopy Abrahamsson, Sara Chen, Jiji Hajj, Bassam Stallinga, Sjoerd Katsov, Alexander Y. Wisniewski, Jan Mizuguchi, Gaku Soulle, Pierre Mueller, Florian Darzacq, Claire Dugast Darzacq, Xavier Wu, Carl Bargmann, Cornelia I. Agard, David A. Dahan, Maxime Gustafsson, Mats G. L. Nat Methods Article Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z-scanning and is often too slow to capture biological events. We report a new aberration-corrected multi-focus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multi-focus system enables high-resolution 3D imaging in multiple colors with single molecule sensitivity, at speeds limited by the camera readout time of a single image. 2012-12-09 2013-01 /pmc/articles/PMC4161287/ /pubmed/23223154 http://dx.doi.org/10.1038/nmeth.2277 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Abrahamsson, Sara Chen, Jiji Hajj, Bassam Stallinga, Sjoerd Katsov, Alexander Y. Wisniewski, Jan Mizuguchi, Gaku Soulle, Pierre Mueller, Florian Darzacq, Claire Dugast Darzacq, Xavier Wu, Carl Bargmann, Cornelia I. Agard, David A. Dahan, Maxime Gustafsson, Mats G. L. Fast and sensitive multi-color 3D imaging using aberration-corrected multi-focus microscopy |
| title | Fast and sensitive multi-color 3D imaging using aberration-corrected
multi-focus microscopy |
| title_full | Fast and sensitive multi-color 3D imaging using aberration-corrected
multi-focus microscopy |
| title_fullStr | Fast and sensitive multi-color 3D imaging using aberration-corrected
multi-focus microscopy |
| title_full_unstemmed | Fast and sensitive multi-color 3D imaging using aberration-corrected
multi-focus microscopy |
| title_short | Fast and sensitive multi-color 3D imaging using aberration-corrected
multi-focus microscopy |
| title_sort | fast and sensitive multi-color 3d imaging using aberration-corrected
multi-focus microscopy |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161287/ https://www.ncbi.nlm.nih.gov/pubmed/23223154 http://dx.doi.org/10.1038/nmeth.2277 |
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