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Extensive Recombination Due to Heteroduplexes Generates Large Amounts of Artificial Gene Fragments during PCR

Artificial recombinants can be generated during PCR when more than two genetically distinct templates coexist in a single PCR reaction. These recombinant amplicons can lead to the false interpretation of genetic diversity and incorrect identification of biological phenotypes that do not exist in viv...

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Detalles Bibliográficos
Autores principales:	Liu, Jia, Song, Hongshuo, Liu, Donglai, Zuo, Tao, Lu, Fengmin, Zhuang, Hui, Gao, Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161356/ https://www.ncbi.nlm.nih.gov/pubmed/25211143 http://dx.doi.org/10.1371/journal.pone.0106658

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