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Eukaryote Culturomics of the Gut Reveals New Species

The repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. We further explored this repertoire using PCR-sequencing and culture in seven individuals living in four tropical countries. A total of 41 microeukaryotes inclu...

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Autores principales: Gouba, Nina, Raoult, Didier, Drancourt, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161381/
https://www.ncbi.nlm.nih.gov/pubmed/25210972
http://dx.doi.org/10.1371/journal.pone.0106994
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author Gouba, Nina
Raoult, Didier
Drancourt, Michel
author_facet Gouba, Nina
Raoult, Didier
Drancourt, Michel
author_sort Gouba, Nina
collection PubMed
description The repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. We further explored this repertoire using PCR-sequencing and culture in seven individuals living in four tropical countries. A total of 41 microeukaryotes including 38 different fungal species and three protists were detected. Four fungal species, Davidiella tassiana, Davidiella sp., Corticiaceae sp., and Penicillium sp., were uniquely detected by culture; 27 fungal species were uniquely detected using PCR-sequencing and Candida albicans, Candida glabrata, Trichosporon asahii, Clavispora lusitaniae, Debaryomyces hansenii, Malassezia restricta, and Malassezia sp. were detected using both molecular and culture methods. Fourteen microeukaryotes were shared by the seven individuals, whereas 27 species were found in only one individual, including 11 species in Amazonia, nine species in Polynesia, five species in India, and two species in Senegal. These data support a worldwide distribution of Malassezia sp., Trichosporon sp., and Candida sp. in the gut mycobiome. Here, 13 fungal species and two protists, Stentor roeseli and Vorticella campanula, were observed for first time in the human gut. This study revealed a previously unsuspected diversity in the repertoire of human gut microeukaryotes, suggesting spots for further exploring this repertoire.
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spelling pubmed-41613812014-09-17 Eukaryote Culturomics of the Gut Reveals New Species Gouba, Nina Raoult, Didier Drancourt, Michel PLoS One Research Article The repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. We further explored this repertoire using PCR-sequencing and culture in seven individuals living in four tropical countries. A total of 41 microeukaryotes including 38 different fungal species and three protists were detected. Four fungal species, Davidiella tassiana, Davidiella sp., Corticiaceae sp., and Penicillium sp., were uniquely detected by culture; 27 fungal species were uniquely detected using PCR-sequencing and Candida albicans, Candida glabrata, Trichosporon asahii, Clavispora lusitaniae, Debaryomyces hansenii, Malassezia restricta, and Malassezia sp. were detected using both molecular and culture methods. Fourteen microeukaryotes were shared by the seven individuals, whereas 27 species were found in only one individual, including 11 species in Amazonia, nine species in Polynesia, five species in India, and two species in Senegal. These data support a worldwide distribution of Malassezia sp., Trichosporon sp., and Candida sp. in the gut mycobiome. Here, 13 fungal species and two protists, Stentor roeseli and Vorticella campanula, were observed for first time in the human gut. This study revealed a previously unsuspected diversity in the repertoire of human gut microeukaryotes, suggesting spots for further exploring this repertoire. Public Library of Science 2014-09-11 /pmc/articles/PMC4161381/ /pubmed/25210972 http://dx.doi.org/10.1371/journal.pone.0106994 Text en © 2014 Gouba et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gouba, Nina
Raoult, Didier
Drancourt, Michel
Eukaryote Culturomics of the Gut Reveals New Species
title Eukaryote Culturomics of the Gut Reveals New Species
title_full Eukaryote Culturomics of the Gut Reveals New Species
title_fullStr Eukaryote Culturomics of the Gut Reveals New Species
title_full_unstemmed Eukaryote Culturomics of the Gut Reveals New Species
title_short Eukaryote Culturomics of the Gut Reveals New Species
title_sort eukaryote culturomics of the gut reveals new species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161381/
https://www.ncbi.nlm.nih.gov/pubmed/25210972
http://dx.doi.org/10.1371/journal.pone.0106994
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