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Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast

Centrosomes play critical roles in the cell division cycle and ciliogenesis. Sfi1 is a centrin-binding protein conserved from yeast to humans. Budding yeast Sfi1 is essential for the initiation of spindle pole body (SPB; yeast centrosome) duplication. However, the recruitment and partitioning of Sfi...

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Autores principales: Lee, I-Ju, Wang, Ning, Hu, Wen, Schott, Kersey, Bähler, Jürg, Giddings, Thomas H., Pringle, John R., Du, Li-Lin, Wu, Jian-Qiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161509/
https://www.ncbi.nlm.nih.gov/pubmed/25031431
http://dx.doi.org/10.1091/mbc.E13-11-0699
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author Lee, I-Ju
Wang, Ning
Hu, Wen
Schott, Kersey
Bähler, Jürg
Giddings, Thomas H.
Pringle, John R.
Du, Li-Lin
Wu, Jian-Qiu
author_facet Lee, I-Ju
Wang, Ning
Hu, Wen
Schott, Kersey
Bähler, Jürg
Giddings, Thomas H.
Pringle, John R.
Du, Li-Lin
Wu, Jian-Qiu
author_sort Lee, I-Ju
collection PubMed
description Centrosomes play critical roles in the cell division cycle and ciliogenesis. Sfi1 is a centrin-binding protein conserved from yeast to humans. Budding yeast Sfi1 is essential for the initiation of spindle pole body (SPB; yeast centrosome) duplication. However, the recruitment and partitioning of Sfi1 to centrosomal structures have never been fully investigated in any organism, and the presumed importance of the conserved tryptophans in the internal repeats of Sfi1 remains untested. Here we report that in fission yeast, instead of doubling abruptly at the initiation of SPB duplication and remaining at a constant level thereafter, Sfi1 is gradually recruited to SPBs throughout the cell cycle. Like an sfi1Δ mutant, a Trp-to-Arg mutant (sfi1-M46) forms monopolar spindles and exhibits mitosis and cytokinesis defects. Sfi1-M46 protein associates preferentially with one of the two daughter SPBs during mitosis, resulting in a failure of new SPB assembly in the SPB receiving insufficient Sfi1. Although all five conserved tryptophans tested are involved in Sfi1 partitioning, the importance of the individual repeats in Sfi1 differs. In summary, our results reveal a link between the conserved tryptophans and Sfi1 partitioning and suggest a revision of the model for SPB assembly.
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spelling pubmed-41615092014-11-30 Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast Lee, I-Ju Wang, Ning Hu, Wen Schott, Kersey Bähler, Jürg Giddings, Thomas H. Pringle, John R. Du, Li-Lin Wu, Jian-Qiu Mol Biol Cell Articles Centrosomes play critical roles in the cell division cycle and ciliogenesis. Sfi1 is a centrin-binding protein conserved from yeast to humans. Budding yeast Sfi1 is essential for the initiation of spindle pole body (SPB; yeast centrosome) duplication. However, the recruitment and partitioning of Sfi1 to centrosomal structures have never been fully investigated in any organism, and the presumed importance of the conserved tryptophans in the internal repeats of Sfi1 remains untested. Here we report that in fission yeast, instead of doubling abruptly at the initiation of SPB duplication and remaining at a constant level thereafter, Sfi1 is gradually recruited to SPBs throughout the cell cycle. Like an sfi1Δ mutant, a Trp-to-Arg mutant (sfi1-M46) forms monopolar spindles and exhibits mitosis and cytokinesis defects. Sfi1-M46 protein associates preferentially with one of the two daughter SPBs during mitosis, resulting in a failure of new SPB assembly in the SPB receiving insufficient Sfi1. Although all five conserved tryptophans tested are involved in Sfi1 partitioning, the importance of the individual repeats in Sfi1 differs. In summary, our results reveal a link between the conserved tryptophans and Sfi1 partitioning and suggest a revision of the model for SPB assembly. The American Society for Cell Biology 2014-09-15 /pmc/articles/PMC4161509/ /pubmed/25031431 http://dx.doi.org/10.1091/mbc.E13-11-0699 Text en © 2014 Lee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Lee, I-Ju
Wang, Ning
Hu, Wen
Schott, Kersey
Bähler, Jürg
Giddings, Thomas H.
Pringle, John R.
Du, Li-Lin
Wu, Jian-Qiu
Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast
title Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast
title_full Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast
title_fullStr Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast
title_full_unstemmed Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast
title_short Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast
title_sort regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein sfi1 in fission yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161509/
https://www.ncbi.nlm.nih.gov/pubmed/25031431
http://dx.doi.org/10.1091/mbc.E13-11-0699
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