Cargando…
A role for eisosomes in maintenance of plasma membrane phosphoinositide levels
The plasma membrane delineates the cell and mediates its communication and material exchange with the environment. Many processes of the plasma membrane occur through interactions of proteins with phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)), which is highly enriched in this membrane and is...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161514/ https://www.ncbi.nlm.nih.gov/pubmed/25057013 http://dx.doi.org/10.1091/mbc.E13-11-0639 |
_version_ | 1782334573588250624 |
---|---|
author | Fröhlich, Florian Christiano, Romain Olson, Daniel K. Alcazar-Roman, Abel DeCamilli, Pietro Walther, Tobias C. |
author_facet | Fröhlich, Florian Christiano, Romain Olson, Daniel K. Alcazar-Roman, Abel DeCamilli, Pietro Walther, Tobias C. |
author_sort | Fröhlich, Florian |
collection | PubMed |
description | The plasma membrane delineates the cell and mediates its communication and material exchange with the environment. Many processes of the plasma membrane occur through interactions of proteins with phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)), which is highly enriched in this membrane and is a key determinant of its identity. Eisosomes function in lateral organization of the plasma membrane, but the molecular function of their major protein subunits, the BAR domain–containing proteins Pil1 and Lsp1, is poorly understood. Here we show that eisosomes interact with the PI(4,5)P(2) phosphatase Inp51/Sjl1, thereby recruiting it to the plasma membrane. Pil1 is essential for plasma membrane localization and function of Inp51 but not for the homologous phosphatidylinositol bisphosphate phosphatases Inp52/Sjl2 and Inp53/Sjl3. Consistent with this, absence of Pil1 increases total and available PI(4,5)P(2) levels at the plasma membrane. On the basis of these findings, we propose a model in which the eisosomes function in maintaining PI(4,5)P(2) levels by Inp51/Sjl1 recruitment. |
format | Online Article Text |
id | pubmed-4161514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41615142014-11-30 A role for eisosomes in maintenance of plasma membrane phosphoinositide levels Fröhlich, Florian Christiano, Romain Olson, Daniel K. Alcazar-Roman, Abel DeCamilli, Pietro Walther, Tobias C. Mol Biol Cell Articles The plasma membrane delineates the cell and mediates its communication and material exchange with the environment. Many processes of the plasma membrane occur through interactions of proteins with phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)), which is highly enriched in this membrane and is a key determinant of its identity. Eisosomes function in lateral organization of the plasma membrane, but the molecular function of their major protein subunits, the BAR domain–containing proteins Pil1 and Lsp1, is poorly understood. Here we show that eisosomes interact with the PI(4,5)P(2) phosphatase Inp51/Sjl1, thereby recruiting it to the plasma membrane. Pil1 is essential for plasma membrane localization and function of Inp51 but not for the homologous phosphatidylinositol bisphosphate phosphatases Inp52/Sjl2 and Inp53/Sjl3. Consistent with this, absence of Pil1 increases total and available PI(4,5)P(2) levels at the plasma membrane. On the basis of these findings, we propose a model in which the eisosomes function in maintaining PI(4,5)P(2) levels by Inp51/Sjl1 recruitment. The American Society for Cell Biology 2014-09-15 /pmc/articles/PMC4161514/ /pubmed/25057013 http://dx.doi.org/10.1091/mbc.E13-11-0639 Text en © 2014 Fröhlich et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Fröhlich, Florian Christiano, Romain Olson, Daniel K. Alcazar-Roman, Abel DeCamilli, Pietro Walther, Tobias C. A role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
title | A role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
title_full | A role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
title_fullStr | A role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
title_full_unstemmed | A role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
title_short | A role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
title_sort | role for eisosomes in maintenance of plasma membrane phosphoinositide levels |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161514/ https://www.ncbi.nlm.nih.gov/pubmed/25057013 http://dx.doi.org/10.1091/mbc.E13-11-0639 |
work_keys_str_mv | AT frohlichflorian aroleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT christianoromain aroleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT olsondanielk aroleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT alcazarromanabel aroleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT decamillipietro aroleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT walthertobiasc aroleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT frohlichflorian roleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT christianoromain roleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT olsondanielk roleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT alcazarromanabel roleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT decamillipietro roleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels AT walthertobiasc roleforeisosomesinmaintenanceofplasmamembranephosphoinositidelevels |