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A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli

Chromatin assembly factor-1 (CAF-1) is a three-subunit protein complex conserved throughout eukaryotes that deposits histones during DNA synthesis. Here we present a novel role for the human p150 subunit in regulating nucleolar macromolecular interactions. Acute depletion of p150 causes redistributi...

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Autores principales: Smith, Corey L., Matheson, Timothy D., Trombly, Daniel J., Sun, Xiaoming, Campeau, Eric, Han, Xuemei, Yates, John R., Kaufman, Paul D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161520/
https://www.ncbi.nlm.nih.gov/pubmed/25057015
http://dx.doi.org/10.1091/mbc.E14-05-1029
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author Smith, Corey L.
Matheson, Timothy D.
Trombly, Daniel J.
Sun, Xiaoming
Campeau, Eric
Han, Xuemei
Yates, John R.
Kaufman, Paul D.
author_facet Smith, Corey L.
Matheson, Timothy D.
Trombly, Daniel J.
Sun, Xiaoming
Campeau, Eric
Han, Xuemei
Yates, John R.
Kaufman, Paul D.
author_sort Smith, Corey L.
collection PubMed
description Chromatin assembly factor-1 (CAF-1) is a three-subunit protein complex conserved throughout eukaryotes that deposits histones during DNA synthesis. Here we present a novel role for the human p150 subunit in regulating nucleolar macromolecular interactions. Acute depletion of p150 causes redistribution of multiple nucleolar proteins and reduces nucleolar association with several repetitive element–containing loci. Of note, a point mutation in a SUMO-interacting motif (SIM) within p150 abolishes nucleolar associations, whereas PCNA or HP1 interaction sites within p150 are not required for these interactions. In addition, acute depletion of SUMO-2 or the SUMO E2 ligase Ubc9 reduces α-satellite DNA association with nucleoli. The nucleolar functions of p150 are separable from its interactions with the other subunits of the CAF-1 complex because an N-terminal fragment of p150 (p150N) that cannot interact with other CAF-1 subunits is sufficient for maintaining nucleolar chromosome and protein associations. Therefore these data define novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus.
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spelling pubmed-41615202014-11-30 A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli Smith, Corey L. Matheson, Timothy D. Trombly, Daniel J. Sun, Xiaoming Campeau, Eric Han, Xuemei Yates, John R. Kaufman, Paul D. Mol Biol Cell Articles Chromatin assembly factor-1 (CAF-1) is a three-subunit protein complex conserved throughout eukaryotes that deposits histones during DNA synthesis. Here we present a novel role for the human p150 subunit in regulating nucleolar macromolecular interactions. Acute depletion of p150 causes redistribution of multiple nucleolar proteins and reduces nucleolar association with several repetitive element–containing loci. Of note, a point mutation in a SUMO-interacting motif (SIM) within p150 abolishes nucleolar associations, whereas PCNA or HP1 interaction sites within p150 are not required for these interactions. In addition, acute depletion of SUMO-2 or the SUMO E2 ligase Ubc9 reduces α-satellite DNA association with nucleoli. The nucleolar functions of p150 are separable from its interactions with the other subunits of the CAF-1 complex because an N-terminal fragment of p150 (p150N) that cannot interact with other CAF-1 subunits is sufficient for maintaining nucleolar chromosome and protein associations. Therefore these data define novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus. The American Society for Cell Biology 2014-09-15 /pmc/articles/PMC4161520/ /pubmed/25057015 http://dx.doi.org/10.1091/mbc.E14-05-1029 Text en © 2014 Smith et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Smith, Corey L.
Matheson, Timothy D.
Trombly, Daniel J.
Sun, Xiaoming
Campeau, Eric
Han, Xuemei
Yates, John R.
Kaufman, Paul D.
A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
title A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
title_full A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
title_fullStr A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
title_full_unstemmed A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
title_short A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
title_sort separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161520/
https://www.ncbi.nlm.nih.gov/pubmed/25057015
http://dx.doi.org/10.1091/mbc.E14-05-1029
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