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The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins

In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is ext...

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Detalles Bibliográficos
Autores principales: Lebendiker, Mario, Danieli, Tsafi, de Marco, Ario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161829/
https://www.ncbi.nlm.nih.gov/pubmed/25178166
http://dx.doi.org/10.1186/1756-0500-7-585
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author Lebendiker, Mario
Danieli, Tsafi
de Marco, Ario
author_facet Lebendiker, Mario
Danieli, Tsafi
de Marco, Ario
author_sort Lebendiker, Mario
collection PubMed
description In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a “quality control” ladder, where the more information given, will lead to a higher ranking of the article. This “quality control” stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-0500-7-585) contains supplementary material, which is available to authorized users.
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spelling pubmed-41618292014-09-13 The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins Lebendiker, Mario Danieli, Tsafi de Marco, Ario BMC Res Notes Correspondence In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a “quality control” ladder, where the more information given, will lead to a higher ranking of the article. This “quality control” stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-0500-7-585) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-01 /pmc/articles/PMC4161829/ /pubmed/25178166 http://dx.doi.org/10.1186/1756-0500-7-585 Text en © Lebendiker et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Correspondence
Lebendiker, Mario
Danieli, Tsafi
de Marco, Ario
The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
title The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
title_full The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
title_fullStr The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
title_full_unstemmed The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
title_short The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
title_sort trip adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
topic Correspondence
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161829/
https://www.ncbi.nlm.nih.gov/pubmed/25178166
http://dx.doi.org/10.1186/1756-0500-7-585
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