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The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins
In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is ext...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161829/ https://www.ncbi.nlm.nih.gov/pubmed/25178166 http://dx.doi.org/10.1186/1756-0500-7-585 |
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author | Lebendiker, Mario Danieli, Tsafi de Marco, Ario |
author_facet | Lebendiker, Mario Danieli, Tsafi de Marco, Ario |
author_sort | Lebendiker, Mario |
collection | PubMed |
description | In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a “quality control” ladder, where the more information given, will lead to a higher ranking of the article. This “quality control” stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-0500-7-585) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4161829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41618292014-09-13 The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins Lebendiker, Mario Danieli, Tsafi de Marco, Ario BMC Res Notes Correspondence In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a “quality control” ladder, where the more information given, will lead to a higher ranking of the article. This “quality control” stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-0500-7-585) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-01 /pmc/articles/PMC4161829/ /pubmed/25178166 http://dx.doi.org/10.1186/1756-0500-7-585 Text en © Lebendiker et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Correspondence Lebendiker, Mario Danieli, Tsafi de Marco, Ario The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
title | The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
title_full | The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
title_fullStr | The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
title_full_unstemmed | The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
title_short | The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
title_sort | trip adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins |
topic | Correspondence |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161829/ https://www.ncbi.nlm.nih.gov/pubmed/25178166 http://dx.doi.org/10.1186/1756-0500-7-585 |
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