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Investigation of olfactory function in a Panx1 knock out mouse model

Pannexin 1 (Panx1), the most extensively investigated member of a channel-forming protein family, is able to form pores conducting molecules up to 1.5 kDa, like ATP, upon activation. In the olfactory epithelium (OE), ATP modulates olfactory responsiveness and plays a role in proliferation and differ...

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Autores principales: Kurtenbach, Stefan, Whyte-Fagundes, Paige, Gelis, Lian, Kurtenbach, Sarah, Brazil, Émerson, Zoidl, Christiane, Hatt, Hanns, Shestopalov, Valery I., Zoidl, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162419/
https://www.ncbi.nlm.nih.gov/pubmed/25309319
http://dx.doi.org/10.3389/fncel.2014.00266
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author Kurtenbach, Stefan
Whyte-Fagundes, Paige
Gelis, Lian
Kurtenbach, Sarah
Brazil, Émerson
Zoidl, Christiane
Hatt, Hanns
Shestopalov, Valery I.
Zoidl, Georg
author_facet Kurtenbach, Stefan
Whyte-Fagundes, Paige
Gelis, Lian
Kurtenbach, Sarah
Brazil, Émerson
Zoidl, Christiane
Hatt, Hanns
Shestopalov, Valery I.
Zoidl, Georg
author_sort Kurtenbach, Stefan
collection PubMed
description Pannexin 1 (Panx1), the most extensively investigated member of a channel-forming protein family, is able to form pores conducting molecules up to 1.5 kDa, like ATP, upon activation. In the olfactory epithelium (OE), ATP modulates olfactory responsiveness and plays a role in proliferation and differentiation of olfactory sensory neurons (OSNs). This process continuously takes place in the OE, as neurons are replaced throughout the whole lifespan. The recent discovery of Panx1 expression in the OE raises the question whether Panx1 mediates ATP release responsible for modulating chemosensory function. In this study, we analyzed pannexin expression in the OE and a possible role of Panx1 in olfactory function using a Panx1(−/−) mouse line with a global ablation of Panx1. This mouse model has been previously used to investigate Panx1 functions in the retina and adult hippocampus. Here, qPCR, in-situ hybridization, and immunohistochemistry (IHC) demonstrated that Panx1 is expressed in axon bundles deriving from sensory neurons of the OE. The localization, distribution, and expression of major olfactory signal transduction proteins were not significantly altered in Panx1(−/−) mice. Further, functional analysis of Panx1(−/−) animals does not reveal any major impairment in odor perception, indicated by electroolfactogram (EOG) measurements and behavioral testing. However, ATP release evoked by potassium gluconate application was reduced in Panx1(−/−) mice. This result is consistent with previous reports on ATP release in isolated erythrocytes and spinal or lumbar cord preparations from Panx1(−/−) mice, suggesting that Panx1 is one of several alternative pathways to release ATP in the olfactory system.
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spelling pubmed-41624192014-10-10 Investigation of olfactory function in a Panx1 knock out mouse model Kurtenbach, Stefan Whyte-Fagundes, Paige Gelis, Lian Kurtenbach, Sarah Brazil, Émerson Zoidl, Christiane Hatt, Hanns Shestopalov, Valery I. Zoidl, Georg Front Cell Neurosci Neuroscience Pannexin 1 (Panx1), the most extensively investigated member of a channel-forming protein family, is able to form pores conducting molecules up to 1.5 kDa, like ATP, upon activation. In the olfactory epithelium (OE), ATP modulates olfactory responsiveness and plays a role in proliferation and differentiation of olfactory sensory neurons (OSNs). This process continuously takes place in the OE, as neurons are replaced throughout the whole lifespan. The recent discovery of Panx1 expression in the OE raises the question whether Panx1 mediates ATP release responsible for modulating chemosensory function. In this study, we analyzed pannexin expression in the OE and a possible role of Panx1 in olfactory function using a Panx1(−/−) mouse line with a global ablation of Panx1. This mouse model has been previously used to investigate Panx1 functions in the retina and adult hippocampus. Here, qPCR, in-situ hybridization, and immunohistochemistry (IHC) demonstrated that Panx1 is expressed in axon bundles deriving from sensory neurons of the OE. The localization, distribution, and expression of major olfactory signal transduction proteins were not significantly altered in Panx1(−/−) mice. Further, functional analysis of Panx1(−/−) animals does not reveal any major impairment in odor perception, indicated by electroolfactogram (EOG) measurements and behavioral testing. However, ATP release evoked by potassium gluconate application was reduced in Panx1(−/−) mice. This result is consistent with previous reports on ATP release in isolated erythrocytes and spinal or lumbar cord preparations from Panx1(−/−) mice, suggesting that Panx1 is one of several alternative pathways to release ATP in the olfactory system. Frontiers Media S.A. 2014-09-12 /pmc/articles/PMC4162419/ /pubmed/25309319 http://dx.doi.org/10.3389/fncel.2014.00266 Text en Copyright © 2014 Kurtenbach, Whyte-Fagundes, Gelis, Kurtenbach, Brazil, Zoidl, Hatt, Shestopalov and Zoidl. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Kurtenbach, Stefan
Whyte-Fagundes, Paige
Gelis, Lian
Kurtenbach, Sarah
Brazil, Émerson
Zoidl, Christiane
Hatt, Hanns
Shestopalov, Valery I.
Zoidl, Georg
Investigation of olfactory function in a Panx1 knock out mouse model
title Investigation of olfactory function in a Panx1 knock out mouse model
title_full Investigation of olfactory function in a Panx1 knock out mouse model
title_fullStr Investigation of olfactory function in a Panx1 knock out mouse model
title_full_unstemmed Investigation of olfactory function in a Panx1 knock out mouse model
title_short Investigation of olfactory function in a Panx1 knock out mouse model
title_sort investigation of olfactory function in a panx1 knock out mouse model
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162419/
https://www.ncbi.nlm.nih.gov/pubmed/25309319
http://dx.doi.org/10.3389/fncel.2014.00266
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