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Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice

BACKGROUND: Graphene oxide (GO) is a promising nanomaterial for potential application in the versatile field of biomedicine. Graphene-based nanomaterials have been reported to modulate the functionality of immune cells in culture and to induce pulmonary inflammation in mice. Evidence pertaining to t...

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Autores principales: Wu, Hsin-Ying, Lin, Kun-Ju, Wang, Ping-Yen, Lin, Chi-Wen, Yang, Hong-Wei, Ma, Chen-Chi M, Lu, Yu-Jen, Jan, Tong-Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162634/
https://www.ncbi.nlm.nih.gov/pubmed/25228804
http://dx.doi.org/10.2147/IJN.S66768
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author Wu, Hsin-Ying
Lin, Kun-Ju
Wang, Ping-Yen
Lin, Chi-Wen
Yang, Hong-Wei
Ma, Chen-Chi M
Lu, Yu-Jen
Jan, Tong-Rong
author_facet Wu, Hsin-Ying
Lin, Kun-Ju
Wang, Ping-Yen
Lin, Chi-Wen
Yang, Hong-Wei
Ma, Chen-Chi M
Lu, Yu-Jen
Jan, Tong-Rong
author_sort Wu, Hsin-Ying
collection PubMed
description BACKGROUND: Graphene oxide (GO) is a promising nanomaterial for potential application in the versatile field of biomedicine. Graphene-based nanomaterials have been reported to modulate the functionality of immune cells in culture and to induce pulmonary inflammation in mice. Evidence pertaining to the interaction between graphene-based nanomaterials and the immune system in vivo remains scarce. The present study investigated the effect of polyethylene glycol-coated GO (PEG-GO) on antigen-specific immunity in vivo. METHODS: BALB/c mice were intravenously administered with a single dose of PEG-GO (0.5 or 1 mg/kg) 1 hour before ovalbumin (OVA) sensitization, and antigen-specific antibody production and splenocyte reactivity were measured 7 days later. RESULTS: Exposure to PEG-GO significantly attenuated the serum level of OVA-specific immunoglobulin E. The production of interferon-γ and interleukin-4 by splenocytes restimulated with OVA in culture was enhanced by treatment with PEG-GO. In addition, PEG-GO augmented the metabolic activity of splenocytes restimulated with OVA but not with the T-cell mitogen concanavalin A. CONCLUSION: Collectively, these results demonstrate that systemic exposure to PEG-GO modulates several aspects of antigen-specific immune responses, including the serum production of immunoglobulin E and T-cell functionality.
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spelling pubmed-41626342014-09-16 Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice Wu, Hsin-Ying Lin, Kun-Ju Wang, Ping-Yen Lin, Chi-Wen Yang, Hong-Wei Ma, Chen-Chi M Lu, Yu-Jen Jan, Tong-Rong Int J Nanomedicine Original Research BACKGROUND: Graphene oxide (GO) is a promising nanomaterial for potential application in the versatile field of biomedicine. Graphene-based nanomaterials have been reported to modulate the functionality of immune cells in culture and to induce pulmonary inflammation in mice. Evidence pertaining to the interaction between graphene-based nanomaterials and the immune system in vivo remains scarce. The present study investigated the effect of polyethylene glycol-coated GO (PEG-GO) on antigen-specific immunity in vivo. METHODS: BALB/c mice were intravenously administered with a single dose of PEG-GO (0.5 or 1 mg/kg) 1 hour before ovalbumin (OVA) sensitization, and antigen-specific antibody production and splenocyte reactivity were measured 7 days later. RESULTS: Exposure to PEG-GO significantly attenuated the serum level of OVA-specific immunoglobulin E. The production of interferon-γ and interleukin-4 by splenocytes restimulated with OVA in culture was enhanced by treatment with PEG-GO. In addition, PEG-GO augmented the metabolic activity of splenocytes restimulated with OVA but not with the T-cell mitogen concanavalin A. CONCLUSION: Collectively, these results demonstrate that systemic exposure to PEG-GO modulates several aspects of antigen-specific immune responses, including the serum production of immunoglobulin E and T-cell functionality. Dove Medical Press 2014-09-08 /pmc/articles/PMC4162634/ /pubmed/25228804 http://dx.doi.org/10.2147/IJN.S66768 Text en © 2014 Wu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wu, Hsin-Ying
Lin, Kun-Ju
Wang, Ping-Yen
Lin, Chi-Wen
Yang, Hong-Wei
Ma, Chen-Chi M
Lu, Yu-Jen
Jan, Tong-Rong
Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_full Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_fullStr Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_full_unstemmed Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_short Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_sort polyethylene glycol-coated graphene oxide attenuates antigen-specific ige production and enhanced antigen-induced t-cell reactivity in ovalbumin-sensitized balb/c mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162634/
https://www.ncbi.nlm.nih.gov/pubmed/25228804
http://dx.doi.org/10.2147/IJN.S66768
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