Cargando…

Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response

Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consumin...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva, Carla C., Mantello, Camila C., Campos, Tatiana, Souza, Livia M., Gonçalves, Paulo S., Souza, Anete P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162974/
https://www.ncbi.nlm.nih.gov/pubmed/25242886
http://dx.doi.org/10.1007/s11032-014-0095-2
_version_ 1782334737253138432
author Silva, Carla C.
Mantello, Camila C.
Campos, Tatiana
Souza, Livia M.
Gonçalves, Paulo S.
Souza, Anete P.
author_facet Silva, Carla C.
Mantello, Camila C.
Campos, Tatiana
Souza, Livia M.
Gonçalves, Paulo S.
Souza, Anete P.
author_sort Silva, Carla C.
collection PubMed
description Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early evaluation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasiliensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-014-0095-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4162974
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-41629742014-09-18 Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response Silva, Carla C. Mantello, Camila C. Campos, Tatiana Souza, Livia M. Gonçalves, Paulo S. Souza, Anete P. Mol Breed Article Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early evaluation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasiliensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-014-0095-2) contains supplementary material, which is available to authorized users. Springer Netherlands 2014-04-29 2014 /pmc/articles/PMC4162974/ /pubmed/25242886 http://dx.doi.org/10.1007/s11032-014-0095-2 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Silva, Carla C.
Mantello, Camila C.
Campos, Tatiana
Souza, Livia M.
Gonçalves, Paulo S.
Souza, Anete P.
Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
title Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
title_full Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
title_fullStr Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
title_full_unstemmed Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
title_short Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
title_sort leaf-, panel- and latex-expressed sequenced tags from the rubber tree (hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162974/
https://www.ncbi.nlm.nih.gov/pubmed/25242886
http://dx.doi.org/10.1007/s11032-014-0095-2
work_keys_str_mv AT silvacarlac leafpanelandlatexexpressedsequencedtagsfromtherubbertreeheveabrasiliensisundercoldstressedandsuboptimalgrowingconditionsthedevelopmentofgenetargetedfunctionalmarkersforstressresponse
AT mantellocamilac leafpanelandlatexexpressedsequencedtagsfromtherubbertreeheveabrasiliensisundercoldstressedandsuboptimalgrowingconditionsthedevelopmentofgenetargetedfunctionalmarkersforstressresponse
AT campostatiana leafpanelandlatexexpressedsequencedtagsfromtherubbertreeheveabrasiliensisundercoldstressedandsuboptimalgrowingconditionsthedevelopmentofgenetargetedfunctionalmarkersforstressresponse
AT souzaliviam leafpanelandlatexexpressedsequencedtagsfromtherubbertreeheveabrasiliensisundercoldstressedandsuboptimalgrowingconditionsthedevelopmentofgenetargetedfunctionalmarkersforstressresponse
AT goncalvespaulos leafpanelandlatexexpressedsequencedtagsfromtherubbertreeheveabrasiliensisundercoldstressedandsuboptimalgrowingconditionsthedevelopmentofgenetargetedfunctionalmarkersforstressresponse
AT souzaanetep leafpanelandlatexexpressedsequencedtagsfromtherubbertreeheveabrasiliensisundercoldstressedandsuboptimalgrowingconditionsthedevelopmentofgenetargetedfunctionalmarkersforstressresponse