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Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity
The gut microbiota is involved in metabolic and immune disorders associated with obesity and type 2 diabetes. We previously demonstrated that prebiotic treatment may significantly improve host health by modulating bacterial species related to the improvement of gut endocrine, barrier and immune func...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163056/ https://www.ncbi.nlm.nih.gov/pubmed/24694712 http://dx.doi.org/10.1038/ismej.2014.45 |
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author | Everard, Amandine Lazarevic, Vladimir Gaïa, Nadia Johansson, Maria Ståhlman, Marcus Backhed, Fredrik Delzenne, Nathalie M Schrenzel, Jacques François, Patrice Cani, Patrice D |
author_facet | Everard, Amandine Lazarevic, Vladimir Gaïa, Nadia Johansson, Maria Ståhlman, Marcus Backhed, Fredrik Delzenne, Nathalie M Schrenzel, Jacques François, Patrice Cani, Patrice D |
author_sort | Everard, Amandine |
collection | PubMed |
description | The gut microbiota is involved in metabolic and immune disorders associated with obesity and type 2 diabetes. We previously demonstrated that prebiotic treatment may significantly improve host health by modulating bacterial species related to the improvement of gut endocrine, barrier and immune functions. An analysis of the gut metagenome is needed to determine which bacterial functions and taxa are responsible for beneficial microbiota–host interactions upon nutritional intervention. We subjected mice to prebiotic (Pre) treatment under physiological (control diet: CT) and pathological conditions (high-fat diet: HFD) for 8 weeks and investigated the production of intestinal antimicrobial peptides and the gut microbiome. HFD feeding significantly decreased the expression of regenerating islet-derived 3-gamma (Reg3g) and phospholipase A2 group-II (PLA2g2) in the jejunum. Prebiotic treatment increased Reg3g expression (by ∼50-fold) and improved intestinal homeostasis as suggested by the increase in the expression of intectin, a key protein involved in intestinal epithelial cell turnover. Deep metagenomic sequencing analysis revealed that HFD and prebiotic treatment significantly affected the gut microbiome at different taxonomic levels. Functional analyses based on the occurrence of clusters of orthologous groups (COGs) of proteins also revealed distinct profiles for the HFD, Pre, HFD-Pre and CT groups. Finally, the gut microbiota modulations induced by prebiotics counteracted HFD-induced inflammation and related metabolic disorders. Thus, we identified novel putative taxa and metabolic functions that may contribute to the development of or protection against the metabolic alterations observed during HFD feeding and HFD-Pre feeding. |
format | Online Article Text |
id | pubmed-4163056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-41630562014-10-03 Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity Everard, Amandine Lazarevic, Vladimir Gaïa, Nadia Johansson, Maria Ståhlman, Marcus Backhed, Fredrik Delzenne, Nathalie M Schrenzel, Jacques François, Patrice Cani, Patrice D ISME J Original Article The gut microbiota is involved in metabolic and immune disorders associated with obesity and type 2 diabetes. We previously demonstrated that prebiotic treatment may significantly improve host health by modulating bacterial species related to the improvement of gut endocrine, barrier and immune functions. An analysis of the gut metagenome is needed to determine which bacterial functions and taxa are responsible for beneficial microbiota–host interactions upon nutritional intervention. We subjected mice to prebiotic (Pre) treatment under physiological (control diet: CT) and pathological conditions (high-fat diet: HFD) for 8 weeks and investigated the production of intestinal antimicrobial peptides and the gut microbiome. HFD feeding significantly decreased the expression of regenerating islet-derived 3-gamma (Reg3g) and phospholipase A2 group-II (PLA2g2) in the jejunum. Prebiotic treatment increased Reg3g expression (by ∼50-fold) and improved intestinal homeostasis as suggested by the increase in the expression of intectin, a key protein involved in intestinal epithelial cell turnover. Deep metagenomic sequencing analysis revealed that HFD and prebiotic treatment significantly affected the gut microbiome at different taxonomic levels. Functional analyses based on the occurrence of clusters of orthologous groups (COGs) of proteins also revealed distinct profiles for the HFD, Pre, HFD-Pre and CT groups. Finally, the gut microbiota modulations induced by prebiotics counteracted HFD-induced inflammation and related metabolic disorders. Thus, we identified novel putative taxa and metabolic functions that may contribute to the development of or protection against the metabolic alterations observed during HFD feeding and HFD-Pre feeding. Nature Publishing Group 2014-10 2014-04-03 /pmc/articles/PMC4163056/ /pubmed/24694712 http://dx.doi.org/10.1038/ismej.2014.45 Text en Copyright © 2014 International Society for Microbial Ecology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Original Article Everard, Amandine Lazarevic, Vladimir Gaïa, Nadia Johansson, Maria Ståhlman, Marcus Backhed, Fredrik Delzenne, Nathalie M Schrenzel, Jacques François, Patrice Cani, Patrice D Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
title | Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
title_full | Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
title_fullStr | Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
title_full_unstemmed | Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
title_short | Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
title_sort | microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163056/ https://www.ncbi.nlm.nih.gov/pubmed/24694712 http://dx.doi.org/10.1038/ismej.2014.45 |
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