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Effect of Trolox addition to cryopreservation media on human sperm motility
Background: Sperm parameters and motion kinetics are affected by cryopreservation. Objective: The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. Materials and Meth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research and Clinical Center for Infertility
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163270/ https://www.ncbi.nlm.nih.gov/pubmed/25242981 |
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author | Minaei, Mohammad Baqer Barbarestani, Mohammad Nekoonam, Saeid Abdolvahabi, Mir Abbas Takzare, Nasrin Asadi, Mohammad Hossein Hedayatpour, Azim Amidi, Fardin |
author_facet | Minaei, Mohammad Baqer Barbarestani, Mohammad Nekoonam, Saeid Abdolvahabi, Mir Abbas Takzare, Nasrin Asadi, Mohammad Hossein Hedayatpour, Azim Amidi, Fardin |
author_sort | Minaei, Mohammad Baqer |
collection | PubMed |
description | Background: Sperm parameters and motion kinetics are affected by cryopreservation. Objective: The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. Materials and Methods: Semen was collected from 20 normal donors and divided into five aliquots prior to cryopreservation. The first aliquot was analyzed by computer-assisted sperm analysis (CASA). Other aliquots were mixed with cryo-protective agent containing 0, 20, 40, and 80 µmol Trolox and treated samples were cryopreserved in liquid nitrogen. After two weeks samples were thawed and sperm motion kinematics was measured by CASA. Percent motility (Mot), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), and amplitude of lateral head displacement (ALH) were compared before and after freeze. Results: Addition of 40µmol Trolox resulted in significantly higher (p<0.05) post thaw VCL, VSL and VAP compared to other groups. Therefore the percentage of post thaw motile spermatozoa were significantly higher (p<0.01). Conclusion: The supplementation of Trolox significantly improved the post-thawed human semen quality, especially progressive motility and average path velocity. |
format | Online Article Text |
id | pubmed-4163270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Research and Clinical Center for Infertility |
record_format | MEDLINE/PubMed |
spelling | pubmed-41632702014-09-19 Effect of Trolox addition to cryopreservation media on human sperm motility Minaei, Mohammad Baqer Barbarestani, Mohammad Nekoonam, Saeid Abdolvahabi, Mir Abbas Takzare, Nasrin Asadi, Mohammad Hossein Hedayatpour, Azim Amidi, Fardin Iran J Reprod Med Original Article Background: Sperm parameters and motion kinetics are affected by cryopreservation. Objective: The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. Materials and Methods: Semen was collected from 20 normal donors and divided into five aliquots prior to cryopreservation. The first aliquot was analyzed by computer-assisted sperm analysis (CASA). Other aliquots were mixed with cryo-protective agent containing 0, 20, 40, and 80 µmol Trolox and treated samples were cryopreserved in liquid nitrogen. After two weeks samples were thawed and sperm motion kinematics was measured by CASA. Percent motility (Mot), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), and amplitude of lateral head displacement (ALH) were compared before and after freeze. Results: Addition of 40µmol Trolox resulted in significantly higher (p<0.05) post thaw VCL, VSL and VAP compared to other groups. Therefore the percentage of post thaw motile spermatozoa were significantly higher (p<0.01). Conclusion: The supplementation of Trolox significantly improved the post-thawed human semen quality, especially progressive motility and average path velocity. Research and Clinical Center for Infertility 2012-03 /pmc/articles/PMC4163270/ /pubmed/25242981 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Minaei, Mohammad Baqer Barbarestani, Mohammad Nekoonam, Saeid Abdolvahabi, Mir Abbas Takzare, Nasrin Asadi, Mohammad Hossein Hedayatpour, Azim Amidi, Fardin Effect of Trolox addition to cryopreservation media on human sperm motility |
title | Effect of Trolox addition to cryopreservation media on human sperm motility |
title_full | Effect of Trolox addition to cryopreservation media on human sperm motility |
title_fullStr | Effect of Trolox addition to cryopreservation media on human sperm motility |
title_full_unstemmed | Effect of Trolox addition to cryopreservation media on human sperm motility |
title_short | Effect of Trolox addition to cryopreservation media on human sperm motility |
title_sort | effect of trolox addition to cryopreservation media on human sperm motility |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163270/ https://www.ncbi.nlm.nih.gov/pubmed/25242981 |
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