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Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family
A Nif3 family protein of Methanocaldococcus jannaschii, MJ0927, is highly conserved from bacteria to humans. Although several structures of bacterial Nif3 proteins are known, no structure representing archaeal Nif3 has yet been reported. The crystal structure of Methanocaldococcus jannaschii MJ0927...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163360/ https://www.ncbi.nlm.nih.gov/pubmed/25243119 http://dx.doi.org/10.1155/2014/171263 |
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author | Chen, Sheng-Chia Huang, Chi-Hung Yang, Chia Shin Kuan, Shu-Min Lin, Ching-Ting Chou, Shan-Ho Chen, Yeh |
author_facet | Chen, Sheng-Chia Huang, Chi-Hung Yang, Chia Shin Kuan, Shu-Min Lin, Ching-Ting Chou, Shan-Ho Chen, Yeh |
author_sort | Chen, Sheng-Chia |
collection | PubMed |
description | A Nif3 family protein of Methanocaldococcus jannaschii, MJ0927, is highly conserved from bacteria to humans. Although several structures of bacterial Nif3 proteins are known, no structure representing archaeal Nif3 has yet been reported. The crystal structure of Methanocaldococcus jannaschii MJ0927 was determined at 2.47 Å resolution to understand the structural differences between the bacterial and archaeal Nif3 proteins. Intriguingly, MJ0927 is found to adopt an unusual assembly comprising a trimer of dimers that forms a cage-like architecture. Electrophoretic mobility-shift assays indicate that MJ0927 binds to both single-stranded and double-stranded DNA. Structural analysis of MJ0927 reveals a positively charged region that can potentially explain its DNA-binding capability. Taken together, these data suggest that MJ0927 adopts a novel quartenary architecture that could play various DNA-binding roles in Methanocaldococcus jannaschii. |
format | Online Article Text |
id | pubmed-4163360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41633602014-09-21 Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family Chen, Sheng-Chia Huang, Chi-Hung Yang, Chia Shin Kuan, Shu-Min Lin, Ching-Ting Chou, Shan-Ho Chen, Yeh Biomed Res Int Research Article A Nif3 family protein of Methanocaldococcus jannaschii, MJ0927, is highly conserved from bacteria to humans. Although several structures of bacterial Nif3 proteins are known, no structure representing archaeal Nif3 has yet been reported. The crystal structure of Methanocaldococcus jannaschii MJ0927 was determined at 2.47 Å resolution to understand the structural differences between the bacterial and archaeal Nif3 proteins. Intriguingly, MJ0927 is found to adopt an unusual assembly comprising a trimer of dimers that forms a cage-like architecture. Electrophoretic mobility-shift assays indicate that MJ0927 binds to both single-stranded and double-stranded DNA. Structural analysis of MJ0927 reveals a positively charged region that can potentially explain its DNA-binding capability. Taken together, these data suggest that MJ0927 adopts a novel quartenary architecture that could play various DNA-binding roles in Methanocaldococcus jannaschii. Hindawi Publishing Corporation 2014 2014-08-28 /pmc/articles/PMC4163360/ /pubmed/25243119 http://dx.doi.org/10.1155/2014/171263 Text en Copyright © 2014 Sheng-Chia Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Sheng-Chia Huang, Chi-Hung Yang, Chia Shin Kuan, Shu-Min Lin, Ching-Ting Chou, Shan-Ho Chen, Yeh Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family |
title | Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family |
title_full | Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family |
title_fullStr | Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family |
title_full_unstemmed | Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family |
title_short | Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family |
title_sort | crystal structure of a conserved hypothetical protein mj0927 from methanocaldococcus jannaschii reveals a novel quaternary assembly in the nif3 family |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163360/ https://www.ncbi.nlm.nih.gov/pubmed/25243119 http://dx.doi.org/10.1155/2014/171263 |
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