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Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addr...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163477/ https://www.ncbi.nlm.nih.gov/pubmed/25243186 http://dx.doi.org/10.1155/2014/836025 |
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author | Liu, Yuan Lv, Ji-Yun Shi, Jian-Fei Yang, Mei Liu, Shu-Hong Li, Zhi-Wei Wang, Hong-Bo Zhang, Shao-Geng Liu, Zhen-Wen Ding, Jin-Biao Xu, Dong-Ping Zhao, Jing-Min |
author_facet | Liu, Yuan Lv, Ji-Yun Shi, Jian-Fei Yang, Mei Liu, Shu-Hong Li, Zhi-Wei Wang, Hong-Bo Zhang, Shao-Geng Liu, Zhen-Wen Ding, Jin-Biao Xu, Dong-Ping Zhao, Jing-Min |
author_sort | Liu, Yuan |
collection | PubMed |
description | Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival. |
format | Online Article Text |
id | pubmed-4163477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41634772014-09-21 Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma Liu, Yuan Lv, Ji-Yun Shi, Jian-Fei Yang, Mei Liu, Shu-Hong Li, Zhi-Wei Wang, Hong-Bo Zhang, Shao-Geng Liu, Zhen-Wen Ding, Jin-Biao Xu, Dong-Ping Zhao, Jing-Min Biomed Res Int Research Article Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival. Hindawi Publishing Corporation 2014 2014-08-27 /pmc/articles/PMC4163477/ /pubmed/25243186 http://dx.doi.org/10.1155/2014/836025 Text en Copyright © 2014 Yuan Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Yuan Lv, Ji-Yun Shi, Jian-Fei Yang, Mei Liu, Shu-Hong Li, Zhi-Wei Wang, Hong-Bo Zhang, Shao-Geng Liu, Zhen-Wen Ding, Jin-Biao Xu, Dong-Ping Zhao, Jing-Min Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma |
title | Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma |
title_full | Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma |
title_fullStr | Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma |
title_full_unstemmed | Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma |
title_short | Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma |
title_sort | targeting the raft-associated akt signaling in hepatocellular carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163477/ https://www.ncbi.nlm.nih.gov/pubmed/25243186 http://dx.doi.org/10.1155/2014/836025 |
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