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Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma

Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addr...

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Autores principales: Liu, Yuan, Lv, Ji-Yun, Shi, Jian-Fei, Yang, Mei, Liu, Shu-Hong, Li, Zhi-Wei, Wang, Hong-Bo, Zhang, Shao-Geng, Liu, Zhen-Wen, Ding, Jin-Biao, Xu, Dong-Ping, Zhao, Jing-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163477/
https://www.ncbi.nlm.nih.gov/pubmed/25243186
http://dx.doi.org/10.1155/2014/836025
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author Liu, Yuan
Lv, Ji-Yun
Shi, Jian-Fei
Yang, Mei
Liu, Shu-Hong
Li, Zhi-Wei
Wang, Hong-Bo
Zhang, Shao-Geng
Liu, Zhen-Wen
Ding, Jin-Biao
Xu, Dong-Ping
Zhao, Jing-Min
author_facet Liu, Yuan
Lv, Ji-Yun
Shi, Jian-Fei
Yang, Mei
Liu, Shu-Hong
Li, Zhi-Wei
Wang, Hong-Bo
Zhang, Shao-Geng
Liu, Zhen-Wen
Ding, Jin-Biao
Xu, Dong-Ping
Zhao, Jing-Min
author_sort Liu, Yuan
collection PubMed
description Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival.
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spelling pubmed-41634772014-09-21 Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma Liu, Yuan Lv, Ji-Yun Shi, Jian-Fei Yang, Mei Liu, Shu-Hong Li, Zhi-Wei Wang, Hong-Bo Zhang, Shao-Geng Liu, Zhen-Wen Ding, Jin-Biao Xu, Dong-Ping Zhao, Jing-Min Biomed Res Int Research Article Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival. Hindawi Publishing Corporation 2014 2014-08-27 /pmc/articles/PMC4163477/ /pubmed/25243186 http://dx.doi.org/10.1155/2014/836025 Text en Copyright © 2014 Yuan Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Yuan
Lv, Ji-Yun
Shi, Jian-Fei
Yang, Mei
Liu, Shu-Hong
Li, Zhi-Wei
Wang, Hong-Bo
Zhang, Shao-Geng
Liu, Zhen-Wen
Ding, Jin-Biao
Xu, Dong-Ping
Zhao, Jing-Min
Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
title Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
title_full Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
title_fullStr Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
title_full_unstemmed Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
title_short Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma
title_sort targeting the raft-associated akt signaling in hepatocellular carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163477/
https://www.ncbi.nlm.nih.gov/pubmed/25243186
http://dx.doi.org/10.1155/2014/836025
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