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Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse

Synaptic plasticity involves the modulation of synaptic connections in response to neuronal activity via multiple pathways. One mechanism modulates synaptic transmission by retrograde signals from the post-synapse that influence the probability of vesicle release in the pre-synapse. Despite its impo...

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Autores principales: Halstead, James M., Lin, Yong Qi, Durraine, Lita, Hamilton, Russell S., Ball, Graeme, Neely, Greg G., Bellen, Hugo J., Davis, Ilan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163661/
https://www.ncbi.nlm.nih.gov/pubmed/25171887
http://dx.doi.org/10.1242/bio.20149027
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author Halstead, James M.
Lin, Yong Qi
Durraine, Lita
Hamilton, Russell S.
Ball, Graeme
Neely, Greg G.
Bellen, Hugo J.
Davis, Ilan
author_facet Halstead, James M.
Lin, Yong Qi
Durraine, Lita
Hamilton, Russell S.
Ball, Graeme
Neely, Greg G.
Bellen, Hugo J.
Davis, Ilan
author_sort Halstead, James M.
collection PubMed
description Synaptic plasticity involves the modulation of synaptic connections in response to neuronal activity via multiple pathways. One mechanism modulates synaptic transmission by retrograde signals from the post-synapse that influence the probability of vesicle release in the pre-synapse. Despite its importance, very few factors required for the expression of retrograde signals, and proper synaptic transmission, have been identified. Here, we identify the conserved RNA binding protein Syncrip as a new factor that modulates the efficiency of vesicle release from the motoneuron and is required for correct synapse structure. We show that syncrip is required genetically and its protein product is detected only in the muscle and not in the motoneuron itself. This unexpected non-autonomy is at least partly explained by the fact that Syncrip modulates retrograde BMP signals from the muscle back to the motoneuron. We show that Syncrip influences the levels of the Bone Morphogenic Protein ligand Glass Bottom Boat from the post-synapse and regulates the pre-synapse. Our results highlight the RNA-binding protein Syncrip as a novel regulator of synaptic output. Given its known role in regulating translation, we propose that Syncrip is important for maintaining a balance between the strength of presynaptic vesicle release and postsynaptic translation.
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spelling pubmed-41636612014-09-23 Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse Halstead, James M. Lin, Yong Qi Durraine, Lita Hamilton, Russell S. Ball, Graeme Neely, Greg G. Bellen, Hugo J. Davis, Ilan Biol Open Research Article Synaptic plasticity involves the modulation of synaptic connections in response to neuronal activity via multiple pathways. One mechanism modulates synaptic transmission by retrograde signals from the post-synapse that influence the probability of vesicle release in the pre-synapse. Despite its importance, very few factors required for the expression of retrograde signals, and proper synaptic transmission, have been identified. Here, we identify the conserved RNA binding protein Syncrip as a new factor that modulates the efficiency of vesicle release from the motoneuron and is required for correct synapse structure. We show that syncrip is required genetically and its protein product is detected only in the muscle and not in the motoneuron itself. This unexpected non-autonomy is at least partly explained by the fact that Syncrip modulates retrograde BMP signals from the muscle back to the motoneuron. We show that Syncrip influences the levels of the Bone Morphogenic Protein ligand Glass Bottom Boat from the post-synapse and regulates the pre-synapse. Our results highlight the RNA-binding protein Syncrip as a novel regulator of synaptic output. Given its known role in regulating translation, we propose that Syncrip is important for maintaining a balance between the strength of presynaptic vesicle release and postsynaptic translation. The Company of Biologists 2014-08-29 /pmc/articles/PMC4163661/ /pubmed/25171887 http://dx.doi.org/10.1242/bio.20149027 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Halstead, James M.
Lin, Yong Qi
Durraine, Lita
Hamilton, Russell S.
Ball, Graeme
Neely, Greg G.
Bellen, Hugo J.
Davis, Ilan
Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
title Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
title_full Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
title_fullStr Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
title_full_unstemmed Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
title_short Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
title_sort syncrip/hnrnp q influences synaptic transmission and regulates bmp signaling at the drosophila neuromuscular synapse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163661/
https://www.ncbi.nlm.nih.gov/pubmed/25171887
http://dx.doi.org/10.1242/bio.20149027
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