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Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling

Znf45l, containing classical C(2)H(2) domains, is a novel member of Zinc finger proteins in zebrafish. In vertebrates, TGF-β signaling plays a critical role in hematopoiesis. Here, we showed that Znf45l is expressed both maternally and zygotically throughout early development. Znf45l-depleted Zebraf...

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Autores principales: Chen, Huijuan, Sun, Huaqin, Tao, Dachang, Yang, Ping, Bian, Shasha, Liu, Yunqiang, Zhang, Sizhong, Ma, Yongxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163844/
https://www.ncbi.nlm.nih.gov/pubmed/24209630
http://dx.doi.org/10.5483/BMBRep.2014.47.1.048
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author Chen, Huijuan
Sun, Huaqin
Tao, Dachang
Yang, Ping
Bian, Shasha
Liu, Yunqiang
Zhang, Sizhong
Ma, Yongxin
author_facet Chen, Huijuan
Sun, Huaqin
Tao, Dachang
Yang, Ping
Bian, Shasha
Liu, Yunqiang
Zhang, Sizhong
Ma, Yongxin
author_sort Chen, Huijuan
collection PubMed
description Znf45l, containing classical C(2)H(2) domains, is a novel member of Zinc finger proteins in zebrafish. In vertebrates, TGF-β signaling plays a critical role in hematopoiesis. Here, we showed that Znf45l is expressed both maternally and zygotically throughout early development. Znf45l-depleted Zebrafish embryos display shorter tails and necrosis with reduced expression of hematopoietic maker genes. Furthermore, we revealed that znf45l locates downstream of TGF-β ligands and maintains normal level of TGF-β receptor type II phosphorylation. In brief, our results indicate that znf45l affects initial hematopoietic development through regulation of TGF-β signaling. [BMB Reports 2014; 47(1): 21-26]
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spelling pubmed-41638442014-09-16 Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling Chen, Huijuan Sun, Huaqin Tao, Dachang Yang, Ping Bian, Shasha Liu, Yunqiang Zhang, Sizhong Ma, Yongxin BMB Rep Research Articles Znf45l, containing classical C(2)H(2) domains, is a novel member of Zinc finger proteins in zebrafish. In vertebrates, TGF-β signaling plays a critical role in hematopoiesis. Here, we showed that Znf45l is expressed both maternally and zygotically throughout early development. Znf45l-depleted Zebrafish embryos display shorter tails and necrosis with reduced expression of hematopoietic maker genes. Furthermore, we revealed that znf45l locates downstream of TGF-β ligands and maintains normal level of TGF-β receptor type II phosphorylation. In brief, our results indicate that znf45l affects initial hematopoietic development through regulation of TGF-β signaling. [BMB Reports 2014; 47(1): 21-26] Korean Society for Biochemistry and Molecular Biology 2014-01 /pmc/articles/PMC4163844/ /pubmed/24209630 http://dx.doi.org/10.5483/BMBRep.2014.47.1.048 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Chen, Huijuan
Sun, Huaqin
Tao, Dachang
Yang, Ping
Bian, Shasha
Liu, Yunqiang
Zhang, Sizhong
Ma, Yongxin
Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
title Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
title_full Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
title_fullStr Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
title_full_unstemmed Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
title_short Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
title_sort znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163844/
https://www.ncbi.nlm.nih.gov/pubmed/24209630
http://dx.doi.org/10.5483/BMBRep.2014.47.1.048
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