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Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1

Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of M...

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Autores principales: Park, Hyunmi, Kim, Youngmi, Park, Deokbum, Jeoung, Dooil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163870/
https://www.ncbi.nlm.nih.gov/pubmed/24286324
http://dx.doi.org/10.5483/BMBRep.2014.47.6.169
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author Park, Hyunmi
Kim, Youngmi
Park, Deokbum
Jeoung, Dooil
author_facet Park, Hyunmi
Kim, Youngmi
Park, Deokbum
Jeoung, Dooil
author_sort Park, Hyunmi
collection PubMed
description Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), negatively regulated the expression of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), showed binding to the promoter sequences of HDAC3. HDAC3 regulated the expression level, and the binding of Ac-H3(K9/14) and Ac-H4(K16) around the MDR1 promoter sequences. The nuclear localization signal domain of HDAC3 was necessary, and sufficient for the binding of HDAC3 to the MDR1 promoter sequences and for conferring sensitivity to microtubule-targeting drugs. [BMB Reports 2014; 47(6): 342-347]
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spelling pubmed-41638702014-09-16 Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 Park, Hyunmi Kim, Youngmi Park, Deokbum Jeoung, Dooil BMB Rep Research Articles Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), negatively regulated the expression of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), showed binding to the promoter sequences of HDAC3. HDAC3 regulated the expression level, and the binding of Ac-H3(K9/14) and Ac-H4(K16) around the MDR1 promoter sequences. The nuclear localization signal domain of HDAC3 was necessary, and sufficient for the binding of HDAC3 to the MDR1 promoter sequences and for conferring sensitivity to microtubule-targeting drugs. [BMB Reports 2014; 47(6): 342-347] Korean Society for Biochemistry and Molecular Biology 2014-06 /pmc/articles/PMC4163870/ /pubmed/24286324 http://dx.doi.org/10.5483/BMBRep.2014.47.6.169 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Park, Hyunmi
Kim, Youngmi
Park, Deokbum
Jeoung, Dooil
Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
title Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
title_full Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
title_fullStr Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
title_full_unstemmed Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
title_short Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
title_sort nuclear localization signal domain of hdac3 is necessary and sufficient for the expression regulation of mdr1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163870/
https://www.ncbi.nlm.nih.gov/pubmed/24286324
http://dx.doi.org/10.5483/BMBRep.2014.47.6.169
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