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Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1
Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of M...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Biochemistry and Molecular Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163870/ https://www.ncbi.nlm.nih.gov/pubmed/24286324 http://dx.doi.org/10.5483/BMBRep.2014.47.6.169 |
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author | Park, Hyunmi Kim, Youngmi Park, Deokbum Jeoung, Dooil |
author_facet | Park, Hyunmi Kim, Youngmi Park, Deokbum Jeoung, Dooil |
author_sort | Park, Hyunmi |
collection | PubMed |
description | Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), negatively regulated the expression of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), showed binding to the promoter sequences of HDAC3. HDAC3 regulated the expression level, and the binding of Ac-H3(K9/14) and Ac-H4(K16) around the MDR1 promoter sequences. The nuclear localization signal domain of HDAC3 was necessary, and sufficient for the binding of HDAC3 to the MDR1 promoter sequences and for conferring sensitivity to microtubule-targeting drugs. [BMB Reports 2014; 47(6): 342-347] |
format | Online Article Text |
id | pubmed-4163870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41638702014-09-16 Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 Park, Hyunmi Kim, Youngmi Park, Deokbum Jeoung, Dooil BMB Rep Research Articles Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), negatively regulated the expression of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3(S424A), showed binding to the promoter sequences of HDAC3. HDAC3 regulated the expression level, and the binding of Ac-H3(K9/14) and Ac-H4(K16) around the MDR1 promoter sequences. The nuclear localization signal domain of HDAC3 was necessary, and sufficient for the binding of HDAC3 to the MDR1 promoter sequences and for conferring sensitivity to microtubule-targeting drugs. [BMB Reports 2014; 47(6): 342-347] Korean Society for Biochemistry and Molecular Biology 2014-06 /pmc/articles/PMC4163870/ /pubmed/24286324 http://dx.doi.org/10.5483/BMBRep.2014.47.6.169 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Park, Hyunmi Kim, Youngmi Park, Deokbum Jeoung, Dooil Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 |
title | Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 |
title_full | Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 |
title_fullStr | Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 |
title_full_unstemmed | Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 |
title_short | Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1 |
title_sort | nuclear localization signal domain of hdac3 is necessary and sufficient for the expression regulation of mdr1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163870/ https://www.ncbi.nlm.nih.gov/pubmed/24286324 http://dx.doi.org/10.5483/BMBRep.2014.47.6.169 |
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