Cargando…

Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells

Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, b...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Su Jeong, Park, Jeen-Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163888/
https://www.ncbi.nlm.nih.gov/pubmed/24286310
http://dx.doi.org/10.5483/BMBRep.2014.47.4.137
_version_ 1782334883307192320
author Lee, Su Jeong
Park, Jeen-Woo
author_facet Lee, Su Jeong
Park, Jeen-Woo
author_sort Lee, Su Jeong
collection PubMed
description Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for antioxidant systems. In this study, we demonstrated that knockdown of IDPc expression by RNA interference enhances UVB-induced apoptosis of immortalized human HaCaT keratinocytes. This effect manifested as DNA fragmentation, changes in cellular redox status, mitochondrial dysfunction, and modulation of apoptotic marker expression. Based on our findings, we suggest that attenuation of IDPc expression may protect skin from UVB-mediated damage, by inducing the apoptosis of UV-damaged cells. [BMB Reports 2014; 47(4): 209-214]
format Online
Article
Text
id pubmed-4163888
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Korean Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-41638882014-09-16 Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells Lee, Su Jeong Park, Jeen-Woo BMB Rep Research Articles Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for antioxidant systems. In this study, we demonstrated that knockdown of IDPc expression by RNA interference enhances UVB-induced apoptosis of immortalized human HaCaT keratinocytes. This effect manifested as DNA fragmentation, changes in cellular redox status, mitochondrial dysfunction, and modulation of apoptotic marker expression. Based on our findings, we suggest that attenuation of IDPc expression may protect skin from UVB-mediated damage, by inducing the apoptosis of UV-damaged cells. [BMB Reports 2014; 47(4): 209-214] Korean Society for Biochemistry and Molecular Biology 2014-04 /pmc/articles/PMC4163888/ /pubmed/24286310 http://dx.doi.org/10.5483/BMBRep.2014.47.4.137 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Lee, Su Jeong
Park, Jeen-Woo
Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells
title Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells
title_full Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells
title_fullStr Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells
title_full_unstemmed Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells
title_short Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP(+)-dependent isocitrate dehydrogenase in HaCaT cells
title_sort enhancement of uvb radiation-mediated apoptosis by knockdown of cytosolic nadp(+)-dependent isocitrate dehydrogenase in hacat cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163888/
https://www.ncbi.nlm.nih.gov/pubmed/24286310
http://dx.doi.org/10.5483/BMBRep.2014.47.4.137
work_keys_str_mv AT leesujeong enhancementofuvbradiationmediatedapoptosisbyknockdownofcytosolicnadpdependentisocitratedehydrogenaseinhacatcells
AT parkjeenwoo enhancementofuvbradiationmediatedapoptosisbyknockdownofcytosolicnadpdependentisocitratedehydrogenaseinhacatcells