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Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163899/ https://www.ncbi.nlm.nih.gov/pubmed/24257118 http://dx.doi.org/10.5483/BMBRep.2014.47.2.090 |
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author | Xie, Hong Hu, Jia Pan, Huan Lou, Yaxin Lv, Ping Chen, Yingyu |
author_facet | Xie, Hong Hu, Jia Pan, Huan Lou, Yaxin Lv, Ping Chen, Yingyu |
author_sort | Xie, Hong |
collection | PubMed |
description | FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non–small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109] |
format | Online Article Text |
id | pubmed-4163899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41638992014-09-16 Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells Xie, Hong Hu, Jia Pan, Huan Lou, Yaxin Lv, Ping Chen, Yingyu BMB Rep Research Articles FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non–small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109] Korean Society for Biochemistry and Molecular Biology 2014-02 /pmc/articles/PMC4163899/ /pubmed/24257118 http://dx.doi.org/10.5483/BMBRep.2014.47.2.090 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Xie, Hong Hu, Jia Pan, Huan Lou, Yaxin Lv, Ping Chen, Yingyu Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells |
title | Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells |
title_full | Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells |
title_fullStr | Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells |
title_full_unstemmed | Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells |
title_short | Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells |
title_sort | adenovirus vector-mediated fam176a overexpression induces cell death in human h1299 non-small cell lung cancer cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163899/ https://www.ncbi.nlm.nih.gov/pubmed/24257118 http://dx.doi.org/10.5483/BMBRep.2014.47.2.090 |
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