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Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells

FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the...

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Autores principales: Xie, Hong, Hu, Jia, Pan, Huan, Lou, Yaxin, Lv, Ping, Chen, Yingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163899/
https://www.ncbi.nlm.nih.gov/pubmed/24257118
http://dx.doi.org/10.5483/BMBRep.2014.47.2.090
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author Xie, Hong
Hu, Jia
Pan, Huan
Lou, Yaxin
Lv, Ping
Chen, Yingyu
author_facet Xie, Hong
Hu, Jia
Pan, Huan
Lou, Yaxin
Lv, Ping
Chen, Yingyu
author_sort Xie, Hong
collection PubMed
description FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non–small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109]
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spelling pubmed-41638992014-09-16 Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells Xie, Hong Hu, Jia Pan, Huan Lou, Yaxin Lv, Ping Chen, Yingyu BMB Rep Research Articles FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non–small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109] Korean Society for Biochemistry and Molecular Biology 2014-02 /pmc/articles/PMC4163899/ /pubmed/24257118 http://dx.doi.org/10.5483/BMBRep.2014.47.2.090 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Xie, Hong
Hu, Jia
Pan, Huan
Lou, Yaxin
Lv, Ping
Chen, Yingyu
Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
title Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
title_full Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
title_fullStr Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
title_full_unstemmed Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
title_short Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
title_sort adenovirus vector-mediated fam176a overexpression induces cell death in human h1299 non-small cell lung cancer cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163899/
https://www.ncbi.nlm.nih.gov/pubmed/24257118
http://dx.doi.org/10.5483/BMBRep.2014.47.2.090
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