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Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?

Meniscal lesions in the avascular zone are still a problem in traumatology. Tissue Engineering approaches with mesenchymal stem cells (MSCs) showed successful regeneration of meniscal defects in the avascular zone. However, in daily clinical practice, a single stage regenerative treatment would be p...

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Autores principales: Zellner, Johannes, Taeger, Christian Dirk, Schaffer, Markus, Roldan, J. Camilo, Loibl, Markus, Mueller, Michael B., Berner, Arne, Krutsch, Werner, Huber, Michaela K. I., Kujat, Richard, Nerlich, Michael, Angele, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164129/
https://www.ncbi.nlm.nih.gov/pubmed/25250325
http://dx.doi.org/10.1155/2014/537686
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author Zellner, Johannes
Taeger, Christian Dirk
Schaffer, Markus
Roldan, J. Camilo
Loibl, Markus
Mueller, Michael B.
Berner, Arne
Krutsch, Werner
Huber, Michaela K. I.
Kujat, Richard
Nerlich, Michael
Angele, Peter
author_facet Zellner, Johannes
Taeger, Christian Dirk
Schaffer, Markus
Roldan, J. Camilo
Loibl, Markus
Mueller, Michael B.
Berner, Arne
Krutsch, Werner
Huber, Michaela K. I.
Kujat, Richard
Nerlich, Michael
Angele, Peter
author_sort Zellner, Johannes
collection PubMed
description Meniscal lesions in the avascular zone are still a problem in traumatology. Tissue Engineering approaches with mesenchymal stem cells (MSCs) showed successful regeneration of meniscal defects in the avascular zone. However, in daily clinical practice, a single stage regenerative treatment would be preferable for meniscus injuries. In particular, clinically applicable bioactive substances or isolated growth factors like platelet-rich plasma (PRP) or bone morphogenic protein 7 (BMP7) are in the focus of interest. In this study, the effects of PRP and BMP7 on the regeneration of avascular meniscal defects were evaluated. In vitro analysis showed that PRP secretes multiple growth factors over a period of 8 days. BMP7 enhances the collagen II deposition in an aggregate culture model of MSCs. However applied to meniscal defects PRP or BMP7 in combination with a hyaluronan collagen composite matrix failed to significantly improve meniscus healing in the avascular zone in a rabbit model after 3 months. Further information of the repair mechanism at the defect site is needed to develop special release systems or carriers for the appropriate application of growth factors to support biological augmentation of meniscus regeneration.
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spelling pubmed-41641292014-09-23 Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone? Zellner, Johannes Taeger, Christian Dirk Schaffer, Markus Roldan, J. Camilo Loibl, Markus Mueller, Michael B. Berner, Arne Krutsch, Werner Huber, Michaela K. I. Kujat, Richard Nerlich, Michael Angele, Peter Biomed Res Int Research Article Meniscal lesions in the avascular zone are still a problem in traumatology. Tissue Engineering approaches with mesenchymal stem cells (MSCs) showed successful regeneration of meniscal defects in the avascular zone. However, in daily clinical practice, a single stage regenerative treatment would be preferable for meniscus injuries. In particular, clinically applicable bioactive substances or isolated growth factors like platelet-rich plasma (PRP) or bone morphogenic protein 7 (BMP7) are in the focus of interest. In this study, the effects of PRP and BMP7 on the regeneration of avascular meniscal defects were evaluated. In vitro analysis showed that PRP secretes multiple growth factors over a period of 8 days. BMP7 enhances the collagen II deposition in an aggregate culture model of MSCs. However applied to meniscal defects PRP or BMP7 in combination with a hyaluronan collagen composite matrix failed to significantly improve meniscus healing in the avascular zone in a rabbit model after 3 months. Further information of the repair mechanism at the defect site is needed to develop special release systems or carriers for the appropriate application of growth factors to support biological augmentation of meniscus regeneration. Hindawi Publishing Corporation 2014 2014-08-31 /pmc/articles/PMC4164129/ /pubmed/25250325 http://dx.doi.org/10.1155/2014/537686 Text en Copyright © 2014 Johannes Zellner et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zellner, Johannes
Taeger, Christian Dirk
Schaffer, Markus
Roldan, J. Camilo
Loibl, Markus
Mueller, Michael B.
Berner, Arne
Krutsch, Werner
Huber, Michaela K. I.
Kujat, Richard
Nerlich, Michael
Angele, Peter
Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?
title Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?
title_full Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?
title_fullStr Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?
title_full_unstemmed Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?
title_short Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?
title_sort are applied growth factors able to mimic the positive effects of mesenchymal stem cells on the regeneration of meniscus in the avascular zone?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164129/
https://www.ncbi.nlm.nih.gov/pubmed/25250325
http://dx.doi.org/10.1155/2014/537686
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