Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel

A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine Serum (FBS) within minutes. The hydrogel was char...

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Detalles Bibliográficos
Autores principales: Shi, Yang, Wang, Jingyu, Wang, Huaimin, Hu, Yanhui, Chen, Xuemei, Yang, Zhimou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164459/
https://www.ncbi.nlm.nih.gov/pubmed/25222132
http://dx.doi.org/10.1371/journal.pone.0106968
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author Shi, Yang
Wang, Jingyu
Wang, Huaimin
Hu, Yanhui
Chen, Xuemei
Yang, Zhimou
author_facet Shi, Yang
Wang, Jingyu
Wang, Huaimin
Hu, Yanhui
Chen, Xuemei
Yang, Zhimou
author_sort Shi, Yang
collection PubMed
description A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine Serum (FBS) within minutes. The hydrogel was characterized by rheology, transmission electron microscopy, and fluorescence emission spectra. Their potential in three dimensional (3D) cell culture was evaluated and the results indicated that the gel with a low concentration of the peptide (0.1 wt%) was suitable for 3D cell culture of 3T3 cells. This study provides an alternative candidate of supramolecular hydrogel for 3D cell culture and cell delivery.
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spelling pubmed-41644592014-09-19 Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel Shi, Yang Wang, Jingyu Wang, Huaimin Hu, Yanhui Chen, Xuemei Yang, Zhimou PLoS One Research Article A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine Serum (FBS) within minutes. The hydrogel was characterized by rheology, transmission electron microscopy, and fluorescence emission spectra. Their potential in three dimensional (3D) cell culture was evaluated and the results indicated that the gel with a low concentration of the peptide (0.1 wt%) was suitable for 3D cell culture of 3T3 cells. This study provides an alternative candidate of supramolecular hydrogel for 3D cell culture and cell delivery. Public Library of Science 2014-09-15 /pmc/articles/PMC4164459/ /pubmed/25222132 http://dx.doi.org/10.1371/journal.pone.0106968 Text en © 2014 Shi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shi, Yang
Wang, Jingyu
Wang, Huaimin
Hu, Yanhui
Chen, Xuemei
Yang, Zhimou
Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel
title Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel
title_full Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel
title_fullStr Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel
title_full_unstemmed Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel
title_short Glutathione-Triggered Formation of a Fmoc-Protected Short Peptide-Based Supramolecular Hydrogel
title_sort glutathione-triggered formation of a fmoc-protected short peptide-based supramolecular hydrogel
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164459/
https://www.ncbi.nlm.nih.gov/pubmed/25222132
http://dx.doi.org/10.1371/journal.pone.0106968
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