Cargando…

A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus

A novel laccase was isolated and purified from fermentation mycelia of mushroom Coprinus comatus with an isolation procedure including three ion-exchange chromatography steps on DEAE-cellulose, CM-cellulose, and Q-Sepharose and one gel-filtration step by fast protein liquid chromatography on Superde...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Shuang, Rong, Cheng-Bo, Kong, Chang, Liu, Yu, Xu, Feng, Miao, Qian-Jiang, Wang, Shou-Xian, Wang, He-Xiang, Zhang, Guo-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164474/
https://www.ncbi.nlm.nih.gov/pubmed/25540778
http://dx.doi.org/10.1155/2014/417461
_version_ 1782334959356215296
author Zhao, Shuang
Rong, Cheng-Bo
Kong, Chang
Liu, Yu
Xu, Feng
Miao, Qian-Jiang
Wang, Shou-Xian
Wang, He-Xiang
Zhang, Guo-Qing
author_facet Zhao, Shuang
Rong, Cheng-Bo
Kong, Chang
Liu, Yu
Xu, Feng
Miao, Qian-Jiang
Wang, Shou-Xian
Wang, He-Xiang
Zhang, Guo-Qing
author_sort Zhao, Shuang
collection PubMed
description A novel laccase was isolated and purified from fermentation mycelia of mushroom Coprinus comatus with an isolation procedure including three ion-exchange chromatography steps on DEAE-cellulose, CM-cellulose, and Q-Sepharose and one gel-filtration step by fast protein liquid chromatography on Superdex 75. The purified enzyme was a monomeric protein with a molecular weight of 64 kDa. It possessed a unique N-terminal amino acid sequence of AIGPVADLKV, which has considerably high sequence similarity with that of other fungal laccases, but is different from that of C. comatus laccases reported. The enzyme manifested an optimal pH value of 2.0 and an optimal temperature of 60(°)C using 2,2′-azinobis(3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) as the substrate. The laccase displayed, at pH 2.0 and 37(°)C, K (m) values of 1.59 mM towards ABTS. It potently suppressed proliferation of tumor cell lines HepG2 and MCF7, and inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC(50) value of 3.46 μM, 4.95 μM, and 5.85 μM, respectively, signifying that it is an antipathogenic protein.
format Online
Article
Text
id pubmed-4164474
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-41644742014-12-24 A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus Zhao, Shuang Rong, Cheng-Bo Kong, Chang Liu, Yu Xu, Feng Miao, Qian-Jiang Wang, Shou-Xian Wang, He-Xiang Zhang, Guo-Qing Biomed Res Int Research Article A novel laccase was isolated and purified from fermentation mycelia of mushroom Coprinus comatus with an isolation procedure including three ion-exchange chromatography steps on DEAE-cellulose, CM-cellulose, and Q-Sepharose and one gel-filtration step by fast protein liquid chromatography on Superdex 75. The purified enzyme was a monomeric protein with a molecular weight of 64 kDa. It possessed a unique N-terminal amino acid sequence of AIGPVADLKV, which has considerably high sequence similarity with that of other fungal laccases, but is different from that of C. comatus laccases reported. The enzyme manifested an optimal pH value of 2.0 and an optimal temperature of 60(°)C using 2,2′-azinobis(3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) as the substrate. The laccase displayed, at pH 2.0 and 37(°)C, K (m) values of 1.59 mM towards ABTS. It potently suppressed proliferation of tumor cell lines HepG2 and MCF7, and inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC(50) value of 3.46 μM, 4.95 μM, and 5.85 μM, respectively, signifying that it is an antipathogenic protein. Hindawi Publishing Corporation 2014 2014-08-28 /pmc/articles/PMC4164474/ /pubmed/25540778 http://dx.doi.org/10.1155/2014/417461 Text en Copyright © 2014 Shuang Zhao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Shuang
Rong, Cheng-Bo
Kong, Chang
Liu, Yu
Xu, Feng
Miao, Qian-Jiang
Wang, Shou-Xian
Wang, He-Xiang
Zhang, Guo-Qing
A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
title A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
title_full A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
title_fullStr A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
title_full_unstemmed A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
title_short A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
title_sort novel laccase with potent antiproliferative and hiv-1 reverse transcriptase inhibitory activities from mycelia of mushroom coprinus comatus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164474/
https://www.ncbi.nlm.nih.gov/pubmed/25540778
http://dx.doi.org/10.1155/2014/417461
work_keys_str_mv AT zhaoshuang anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT rongchengbo anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT kongchang anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT liuyu anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT xufeng anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT miaoqianjiang anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT wangshouxian anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT wanghexiang anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT zhangguoqing anovellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT zhaoshuang novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT rongchengbo novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT kongchang novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT liuyu novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT xufeng novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT miaoqianjiang novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT wangshouxian novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT wanghexiang novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus
AT zhangguoqing novellaccasewithpotentantiproliferativeandhiv1reversetranscriptaseinhibitoryactivitiesfrommyceliaofmushroomcoprinuscomatus