Cargando…
Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells
The purpose of this study was to investigate if PPARγ plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone (α-MSH), α-MSH+retinol, α-MSH+GW9662 (PPARγ antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164518/ https://www.ncbi.nlm.nih.gov/pubmed/25250328 http://dx.doi.org/10.1155/2014/695797 |
_version_ | 1782334965572173824 |
---|---|
author | Chen, Jiun-Han Chang, Junn-Liang Chen, Pei-Ru Chuang, Yun-Ju Tang, Shih-Tsang Pan, Shwu-Fen Lin, Tzer-Bin Chen, Kang-Hua Chen, Mei-Jung |
author_facet | Chen, Jiun-Han Chang, Junn-Liang Chen, Pei-Ru Chuang, Yun-Ju Tang, Shih-Tsang Pan, Shwu-Fen Lin, Tzer-Bin Chen, Kang-Hua Chen, Mei-Jung |
author_sort | Chen, Jiun-Han |
collection | PubMed |
description | The purpose of this study was to investigate if PPARγ plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone (α-MSH), α-MSH+retinol, α-MSH+GW9662 (PPARγ antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco's modified Eagle's medium (DMEM) for 48 hrs. To initiate the melanogenesis, cells in all α-MSH groups were cultured in medium containing α-MSH (10 nM) for 48 hrs. Cells were treated simultaneously with retinol (5 μM) in the α-MSH+retinol group. Instead of retinol, GW9662 (10 μM) was cocultured in the α-MSH+GW9662 group. Cells in the final group were cultured in the DMEM with GW9662. All the analyses were carried out 48 hours after treatments. The α-MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis. These α-MSH-induced changes were prevented either by retinol or by GW9662. Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that α-MSH treatment raised the activity of SOD which was dependent on PPARγ level. According to our results, the α-MSH-induced melanogenesis was PPARγ dependent, which also modulated the expression of SOD. |
format | Online Article Text |
id | pubmed-4164518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41645182014-09-23 Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells Chen, Jiun-Han Chang, Junn-Liang Chen, Pei-Ru Chuang, Yun-Ju Tang, Shih-Tsang Pan, Shwu-Fen Lin, Tzer-Bin Chen, Kang-Hua Chen, Mei-Jung Biomed Res Int Research Article The purpose of this study was to investigate if PPARγ plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone (α-MSH), α-MSH+retinol, α-MSH+GW9662 (PPARγ antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco's modified Eagle's medium (DMEM) for 48 hrs. To initiate the melanogenesis, cells in all α-MSH groups were cultured in medium containing α-MSH (10 nM) for 48 hrs. Cells were treated simultaneously with retinol (5 μM) in the α-MSH+retinol group. Instead of retinol, GW9662 (10 μM) was cocultured in the α-MSH+GW9662 group. Cells in the final group were cultured in the DMEM with GW9662. All the analyses were carried out 48 hours after treatments. The α-MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis. These α-MSH-induced changes were prevented either by retinol or by GW9662. Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that α-MSH treatment raised the activity of SOD which was dependent on PPARγ level. According to our results, the α-MSH-induced melanogenesis was PPARγ dependent, which also modulated the expression of SOD. Hindawi Publishing Corporation 2014 2014-08-28 /pmc/articles/PMC4164518/ /pubmed/25250328 http://dx.doi.org/10.1155/2014/695797 Text en Copyright © 2014 Jiun-Han Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Jiun-Han Chang, Junn-Liang Chen, Pei-Ru Chuang, Yun-Ju Tang, Shih-Tsang Pan, Shwu-Fen Lin, Tzer-Bin Chen, Kang-Hua Chen, Mei-Jung Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells |
title | Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells |
title_full | Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells |
title_fullStr | Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells |
title_full_unstemmed | Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells |
title_short | Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells |
title_sort | inhibition of peroxisome proliferator-activated receptor gamma prevents the melanogenesis in murine b16/f10 melanoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164518/ https://www.ncbi.nlm.nih.gov/pubmed/25250328 http://dx.doi.org/10.1155/2014/695797 |
work_keys_str_mv | AT chenjiunhan inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT changjunnliang inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT chenpeiru inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT chuangyunju inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT tangshihtsang inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT panshwufen inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT lintzerbin inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT chenkanghua inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells AT chenmeijung inhibitionofperoxisomeproliferatoractivatedreceptorgammapreventsthemelanogenesisinmurineb16f10melanomacells |