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Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus
The purpose of this study was to investigate the localization of cells containing the calcium-binding proteins (CBPs) calbindin D28K (CB), calretinin (CR), and parvalbumin (PV) in the superior colliculus (SC) of the bat using immunocytochemistry. CB-immunoreactive (IR) cells formed a laminar tier wi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164697/ https://www.ncbi.nlm.nih.gov/pubmed/25320408 http://dx.doi.org/10.1267/ahc.14004 |
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author | Jeong, Se-Jin Kim, Hyun-Ho Lee, Won-Sig Jeon, Chang-Jin |
author_facet | Jeong, Se-Jin Kim, Hyun-Ho Lee, Won-Sig Jeon, Chang-Jin |
author_sort | Jeong, Se-Jin |
collection | PubMed |
description | The purpose of this study was to investigate the localization of cells containing the calcium-binding proteins (CBPs) calbindin D28K (CB), calretinin (CR), and parvalbumin (PV) in the superior colliculus (SC) of the bat using immunocytochemistry. CB-immunoreactive (IR) cells formed a laminar tier within the upper superficial gray layer (SGL), while CR-IR cells were widely distributed within the optic layer (OL). Scattered CR-IR cells were also found within the intermediate gray, white, and deep gray layers. By contrast, PV-IR cells formed a laminar tier within the lower SGL and upper OL. Scattered PV-IR cells were also found throughout the intermediate layers, but without a specific laminar pattern. The CBP-IR cells varied in size and morphology: While most of the CB-IR cells in the superficial layers were small round or oval cells, most CR-IR cells in the intermediate and deep layers were large stellate cells. By contrast, PV-IR cells were small to large in size and included round or oval, stellate, vertical fusiform, and horizontal cells. The average diameters of the CB-, CR-, and PV-IR cells were 11.59, 17.17, and 12.60 μm, respectively. Double-immunofluorescence revealed that the percentage of co-localization with GABA-IR cells was 0.0, 0.0, and 10.27% of CB-, CR-, and PV-IR cells, respectively. These results indicate that CBP distribution patterns in the bat SC are unique compared with other mammalian SCs, which suggest functional diversity of these proteins in visually guided behaviors. |
format | Online Article Text |
id | pubmed-4164697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY |
record_format | MEDLINE/PubMed |
spelling | pubmed-41646972014-10-15 Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus Jeong, Se-Jin Kim, Hyun-Ho Lee, Won-Sig Jeon, Chang-Jin Acta Histochem Cytochem Regular Article The purpose of this study was to investigate the localization of cells containing the calcium-binding proteins (CBPs) calbindin D28K (CB), calretinin (CR), and parvalbumin (PV) in the superior colliculus (SC) of the bat using immunocytochemistry. CB-immunoreactive (IR) cells formed a laminar tier within the upper superficial gray layer (SGL), while CR-IR cells were widely distributed within the optic layer (OL). Scattered CR-IR cells were also found within the intermediate gray, white, and deep gray layers. By contrast, PV-IR cells formed a laminar tier within the lower SGL and upper OL. Scattered PV-IR cells were also found throughout the intermediate layers, but without a specific laminar pattern. The CBP-IR cells varied in size and morphology: While most of the CB-IR cells in the superficial layers were small round or oval cells, most CR-IR cells in the intermediate and deep layers were large stellate cells. By contrast, PV-IR cells were small to large in size and included round or oval, stellate, vertical fusiform, and horizontal cells. The average diameters of the CB-, CR-, and PV-IR cells were 11.59, 17.17, and 12.60 μm, respectively. Double-immunofluorescence revealed that the percentage of co-localization with GABA-IR cells was 0.0, 0.0, and 10.27% of CB-, CR-, and PV-IR cells, respectively. These results indicate that CBP distribution patterns in the bat SC are unique compared with other mammalian SCs, which suggest functional diversity of these proteins in visually guided behaviors. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2014-06-28 2014-06-26 /pmc/articles/PMC4164697/ /pubmed/25320408 http://dx.doi.org/10.1267/ahc.14004 Text en 2014 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Jeong, Se-Jin Kim, Hyun-Ho Lee, Won-Sig Jeon, Chang-Jin Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus |
title | Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus |
title_full | Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus |
title_fullStr | Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus |
title_full_unstemmed | Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus |
title_short | Immunocytochemical Localization of Calbindin D28K, Calretinin, and Parvalbumin in Bat Superior Colliculus |
title_sort | immunocytochemical localization of calbindin d28k, calretinin, and parvalbumin in bat superior colliculus |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164697/ https://www.ncbi.nlm.nih.gov/pubmed/25320408 http://dx.doi.org/10.1267/ahc.14004 |
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