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Effect of oxytocin injection to milching buffaloes on its content & stability in milk

BACKGROUND & OBJECTIVES: Oxytocin (OT) injections to milch cattle for milk letdown have become a common practice amongst dairy farmers in India. Although there is no reported evidence, it is widely presumed that long term consumption of such milk leads to adverse health consequences. However, th...

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Autores principales: Pullakhandam, Raghu, Palika, Ravindranadh, Vemula, Sudershan Rao, Polasa, Kalpagam, Boindala, Sesikeran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165007/
https://www.ncbi.nlm.nih.gov/pubmed/25109729
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author Pullakhandam, Raghu
Palika, Ravindranadh
Vemula, Sudershan Rao
Polasa, Kalpagam
Boindala, Sesikeran
author_facet Pullakhandam, Raghu
Palika, Ravindranadh
Vemula, Sudershan Rao
Polasa, Kalpagam
Boindala, Sesikeran
author_sort Pullakhandam, Raghu
collection PubMed
description BACKGROUND & OBJECTIVES: Oxytocin (OT) injections to milch cattle for milk letdown have become a common practice amongst dairy farmers in India. Although there is no reported evidence, it is widely presumed that long term consumption of such milk leads to adverse health consequences. However, there is no information on the effect of exogenous OT injections on milk OT content and its stability during heating and gastrointestinal digestion. This study was carried out to determine the OT content in milk samples given by buffaloes with and without OT injections and to assess the stability of OT in the milk. METHODS: Milk samples from milch buffaloes (Murrah buffalo) were collected from local farmers with (n=121) or without (n=120) exogenous OT injections during 3 to 5 months of lactation period. The OT content of milk samples was estimated by competitive enzyme immunoassay (EIA). The thermal and digestive stability of OT was assessed by in silico and in vitro digestion methods. RESULTS: The OT content of the milk samples was similar regardless of OT injections used. Further, OT was found to be stable to heat treatment and gastric pepsin digestion while it was rapidly digested during the simulated intestinal digestion. Reduced OT was digested by pepsin, implying that internal disulphide bridge of OT rendered the peptide resistant to peptic digestion. On the other hand, phenylmethylsulphonyl fluoride (PMSF), a serine protease inhibitor, abrogated the pancreatin induced digestion of OT. INTERPRETATION & CONCLUSIONS: These findings suggest that exogenous OT injections do not influence its content in milk. Further, OT present in milk is rapidly degraded during intestinal digestion, ruling out its intestinal absorption and associated adverse health consequences, if any.
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spelling pubmed-41650072014-10-28 Effect of oxytocin injection to milching buffaloes on its content & stability in milk Pullakhandam, Raghu Palika, Ravindranadh Vemula, Sudershan Rao Polasa, Kalpagam Boindala, Sesikeran Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Oxytocin (OT) injections to milch cattle for milk letdown have become a common practice amongst dairy farmers in India. Although there is no reported evidence, it is widely presumed that long term consumption of such milk leads to adverse health consequences. However, there is no information on the effect of exogenous OT injections on milk OT content and its stability during heating and gastrointestinal digestion. This study was carried out to determine the OT content in milk samples given by buffaloes with and without OT injections and to assess the stability of OT in the milk. METHODS: Milk samples from milch buffaloes (Murrah buffalo) were collected from local farmers with (n=121) or without (n=120) exogenous OT injections during 3 to 5 months of lactation period. The OT content of milk samples was estimated by competitive enzyme immunoassay (EIA). The thermal and digestive stability of OT was assessed by in silico and in vitro digestion methods. RESULTS: The OT content of the milk samples was similar regardless of OT injections used. Further, OT was found to be stable to heat treatment and gastric pepsin digestion while it was rapidly digested during the simulated intestinal digestion. Reduced OT was digested by pepsin, implying that internal disulphide bridge of OT rendered the peptide resistant to peptic digestion. On the other hand, phenylmethylsulphonyl fluoride (PMSF), a serine protease inhibitor, abrogated the pancreatin induced digestion of OT. INTERPRETATION & CONCLUSIONS: These findings suggest that exogenous OT injections do not influence its content in milk. Further, OT present in milk is rapidly degraded during intestinal digestion, ruling out its intestinal absorption and associated adverse health consequences, if any. Medknow Publications & Media Pvt Ltd 2014-06 /pmc/articles/PMC4165007/ /pubmed/25109729 Text en Copyright: © Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pullakhandam, Raghu
Palika, Ravindranadh
Vemula, Sudershan Rao
Polasa, Kalpagam
Boindala, Sesikeran
Effect of oxytocin injection to milching buffaloes on its content & stability in milk
title Effect of oxytocin injection to milching buffaloes on its content & stability in milk
title_full Effect of oxytocin injection to milching buffaloes on its content & stability in milk
title_fullStr Effect of oxytocin injection to milching buffaloes on its content & stability in milk
title_full_unstemmed Effect of oxytocin injection to milching buffaloes on its content & stability in milk
title_short Effect of oxytocin injection to milching buffaloes on its content & stability in milk
title_sort effect of oxytocin injection to milching buffaloes on its content & stability in milk
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165007/
https://www.ncbi.nlm.nih.gov/pubmed/25109729
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