Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking

We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the c...

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Autores principales: Sekiguchi, Hiroshi, Suzuki, Yasuhito, Nishino, Yuri, Kobayashi, Suzuko, Shimoyama, Yoshiko, Cai, Weiyan, Nagata, Kenji, Okada, Masato, Ichiyanagi, Kouhei, Ohta, Noboru, Yagi, Naoto, Miyazawa, Atsuo, Kubo, Tai, Sasaki, Yuji C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165275/
https://www.ncbi.nlm.nih.gov/pubmed/25223459
http://dx.doi.org/10.1038/srep06384
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author Sekiguchi, Hiroshi
Suzuki, Yasuhito
Nishino, Yuri
Kobayashi, Suzuko
Shimoyama, Yoshiko
Cai, Weiyan
Nagata, Kenji
Okada, Masato
Ichiyanagi, Kouhei
Ohta, Noboru
Yagi, Naoto
Miyazawa, Atsuo
Kubo, Tai
Sasaki, Yuji C.
author_facet Sekiguchi, Hiroshi
Suzuki, Yasuhito
Nishino, Yuri
Kobayashi, Suzuko
Shimoyama, Yoshiko
Cai, Weiyan
Nagata, Kenji
Okada, Masato
Ichiyanagi, Kouhei
Ohta, Noboru
Yagi, Naoto
Miyazawa, Atsuo
Kubo, Tai
Sasaki, Yuji C.
author_sort Sekiguchi, Hiroshi
collection PubMed
description We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or α-bungarotoxin (αBtx), with views from two rotational axes. Our findings indicate that specific motion patterns represented as biaxial angular motion maps are associated with channel function in real time and on an atomic scale.
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spelling pubmed-41652752014-09-22 Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking Sekiguchi, Hiroshi Suzuki, Yasuhito Nishino, Yuri Kobayashi, Suzuko Shimoyama, Yoshiko Cai, Weiyan Nagata, Kenji Okada, Masato Ichiyanagi, Kouhei Ohta, Noboru Yagi, Naoto Miyazawa, Atsuo Kubo, Tai Sasaki, Yuji C. Sci Rep Article We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or α-bungarotoxin (αBtx), with views from two rotational axes. Our findings indicate that specific motion patterns represented as biaxial angular motion maps are associated with channel function in real time and on an atomic scale. Nature Publishing Group 2014-09-16 /pmc/articles/PMC4165275/ /pubmed/25223459 http://dx.doi.org/10.1038/srep06384 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sekiguchi, Hiroshi
Suzuki, Yasuhito
Nishino, Yuri
Kobayashi, Suzuko
Shimoyama, Yoshiko
Cai, Weiyan
Nagata, Kenji
Okada, Masato
Ichiyanagi, Kouhei
Ohta, Noboru
Yagi, Naoto
Miyazawa, Atsuo
Kubo, Tai
Sasaki, Yuji C.
Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
title Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
title_full Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
title_fullStr Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
title_full_unstemmed Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
title_short Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
title_sort real time ligand-induced motion mappings of achbp and nachr using x-ray single molecule tracking
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165275/
https://www.ncbi.nlm.nih.gov/pubmed/25223459
http://dx.doi.org/10.1038/srep06384
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