16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles

BACKGROUND: Sample storage conditions, extraction methods, PCR primers, and parameters are major factors that affect metagenomics analysis based on microbial 16S rRNA gene sequencing. Most published studies were limited to the comparison of only one or two types of these factors. Systematic multi-fa...

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Detalles Bibliográficos
Autores principales: Hang, Jun, Desai, Valmik, Zavaljevski, Nela, Yang, Yu, Lin, Xiaoxu, Satya, Ravi Vijaya, Martinez, Luis J, Blaylock, Jason M, Jarman, Richard G, Thomas, Stephen J, Kuschner, Robert A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165438/
https://www.ncbi.nlm.nih.gov/pubmed/25228989
http://dx.doi.org/10.1186/2049-2618-2-31

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