Comprehensive analysis of mutually exclusive alternative splicing in C. elegans

Mutually exclusive selection of one exon in a cluster of exons is a rare form of alternative pre-mRNA splicing, yet suggests strict regulation. However, the repertoires of regulation mechanisms for the mutually exclusive (ME) splicing in vivo are still unknown. Here, we experimentally explore putati...

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Detalles Bibliográficos
Autores principales: Kuroyanagi, Hidehito, Takei, Satomi, Suzuki, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165533/
https://www.ncbi.nlm.nih.gov/pubmed/25254147
http://dx.doi.org/10.4161/worm.28459
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author Kuroyanagi, Hidehito
Takei, Satomi
Suzuki, Yutaka
author_facet Kuroyanagi, Hidehito
Takei, Satomi
Suzuki, Yutaka
author_sort Kuroyanagi, Hidehito
collection PubMed
description Mutually exclusive selection of one exon in a cluster of exons is a rare form of alternative pre-mRNA splicing, yet suggests strict regulation. However, the repertoires of regulation mechanisms for the mutually exclusive (ME) splicing in vivo are still unknown. Here, we experimentally explore putative ME exons in C. elegans to demonstrate that 29 ME exon clusters in 27 genes are actually selected in a mutually exclusive manner. Twenty-two of the clusters consist of homologous ME exons. Five clusters have too short intervening introns to be excised between the ME exons. Fidelity of ME splicing relies at least in part on nonsense-mediated mRNA decay for 14 clusters. These results thus characterize all the repertoires of ME splicing in this organism.
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spelling pubmed-41655332015-03-31 Comprehensive analysis of mutually exclusive alternative splicing in C. elegans Kuroyanagi, Hidehito Takei, Satomi Suzuki, Yutaka Worm Short Communication Mutually exclusive selection of one exon in a cluster of exons is a rare form of alternative pre-mRNA splicing, yet suggests strict regulation. However, the repertoires of regulation mechanisms for the mutually exclusive (ME) splicing in vivo are still unknown. Here, we experimentally explore putative ME exons in C. elegans to demonstrate that 29 ME exon clusters in 27 genes are actually selected in a mutually exclusive manner. Twenty-two of the clusters consist of homologous ME exons. Five clusters have too short intervening introns to be excised between the ME exons. Fidelity of ME splicing relies at least in part on nonsense-mediated mRNA decay for 14 clusters. These results thus characterize all the repertoires of ME splicing in this organism. Landes Bioscience 2014-03-31 /pmc/articles/PMC4165533/ /pubmed/25254147 http://dx.doi.org/10.4161/worm.28459 Text en Copyright © 2014 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Short Communication
Kuroyanagi, Hidehito
Takei, Satomi
Suzuki, Yutaka
Comprehensive analysis of mutually exclusive alternative splicing in C. elegans
title Comprehensive analysis of mutually exclusive alternative splicing in C. elegans
title_full Comprehensive analysis of mutually exclusive alternative splicing in C. elegans
title_fullStr Comprehensive analysis of mutually exclusive alternative splicing in C. elegans
title_full_unstemmed Comprehensive analysis of mutually exclusive alternative splicing in C. elegans
title_short Comprehensive analysis of mutually exclusive alternative splicing in C. elegans
title_sort comprehensive analysis of mutually exclusive alternative splicing in c. elegans
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165533/
https://www.ncbi.nlm.nih.gov/pubmed/25254147
http://dx.doi.org/10.4161/worm.28459
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