Genotypic and phenotypic detection of efflux pump in Rhodococcus equi

The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R....

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Detalles Bibliográficos
Autores principales: Gressler, Letícia Trevisan, de Vargas, Agueda Castagna, da Costa, Mateus Matiuzzi, Pötter, Luciana, da Silveira, Bibiana Petri, Sangioni, Luis Antônio, de Avila Botton, Sônia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166297/
https://www.ncbi.nlm.nih.gov/pubmed/25242956
Descripción
Sumario:The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.

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